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Induction of a chemoattractive proinflammatory cytokine response after stimulation of keratinocytes with Propionibacterium acnes and coproporphyrin III.

AbstractBACKGROUND:
The inflammation in acne vulgaris is widely thought to be induced by an immunological reaction, but the role of Propionibacterium acnes is unclear.
OBJECTIVES:
To examine the local host response mechanism of a keratinocyte cell line 3 h and 6 h after stimulation with viable and heat-killed P. acnes.
METHODS:
The quantitative expression of cytokines was measured at the mRNA level by real-time reverse transcription-polymerase chain reaction.
RESULTS:
The coincubation of a keratinocyte cell line with viable, but not heat-killed, P. acnes modulated an adequate cytokine response for interleukin (IL)-1beta, granulocyte/macrophage colony-stimulating factor and IL-8. High-performance liquid chromatographic analysis of the in vivo porphyrin pattern secreted by P. acnes revealed a predominance of coproporphyrin III in acne lesions. This same porphyrin fraction also modestly induced IL-8 expression by keratinocytes.
CONCLUSIONS:
This cytokine pattern may favour a chemotactic response and implicates P. acnes and coproporphyrin III in the recruitment of inflammatory cells to the site of infection and in the development of acne lesions.
AuthorsM Schaller, M Loewenstein, C Borelli, K Jacob, M Vogeser, W H C Burgdorf, G Plewig
JournalThe British journal of dermatology (Br J Dermatol) Vol. 153 Issue 1 Pg. 66-71 (Jul 2005) ISSN: 0007-0963 [Print] England
PMID16029328 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Bacterial
  • Chemotactic Factors
  • Coproporphyrins
  • Cytokines
  • Inflammation Mediators
  • RNA, Messenger
  • coproporphyrin III
Topics
  • Acne Vulgaris (immunology, metabolism, microbiology)
  • Adolescent
  • Adult
  • Antigens, Bacterial (immunology)
  • Cell Line
  • Chemotactic Factors (immunology)
  • Coproporphyrins (biosynthesis, immunology)
  • Cytokines (biosynthesis, genetics, immunology)
  • Humans
  • Inflammation Mediators (metabolism)
  • Keratinocytes (immunology)
  • Propionibacterium acnes (immunology)
  • RNA, Messenger (genetics)
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction (methods)
  • Skin (metabolism)

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