Effect of cysteine protease inhibitor Ep-475 on TNF-alpha-independent cyclophosphamide-induced apoptosis in mouse lymphosarcoma LS cells.

Abstract

Cyclophosphamide 1.5-2.0-fold increased activity of cathepsins B and L in tumor tissue of mouse lymphosarcoma LS and caused tumor regression. The effect was most pronounced on day 5 after treatment. Twofold treatment with a selective cathepsin inhibitor Ep-475 slightly stimulated tumor growth in control mice and significantly reduced the antitumor effect of cyclophosphamide. Lysosomal cysteine proteases cathepsins B and L are involved, but do not play a key role in TNF-alpha-independent apoptosis in LS cells induced by cyclophosphamide.

• Authors: S Ya Zhanaeva, T A Korolenko, O M Khoshchenko, V K Spiridonov, V P Nikolin, V I Kaledin
• Journal: Bulletin of experimental biology and medicine (Bull Exp Biol Med) Vol. 139 Issue 2 Pg. 186-9 (Feb 2005) ISSN: 0007-4888 [Print] United States
• PMID: 16027802 (Publication Type: Journal Article)

Chemical References

• Cysteine Proteinase Inhibitors
• Tumor Necrosis Factor-alpha
• N-(N-(3-carboxyoxirane-2-carbonyl)leucyl)isoamylamine
• Cyclophosphamide
• Cathepsins
• Cysteine Endopeptidases
• Cathepsin B
• Cathepsin L
• Ctsl protein, mouse
• Leucine

Topics

• Animals
• Apoptosis
• Cathepsin B (antagonists & inhibitors)
• Cathepsin L
• Cathepsins (antagonists & inhibitors)
• Cyclophosphamide (pharmacology)
• Cysteine Endopeptidases
• Cysteine Proteinase Inhibitors (pharmacology)
• Leucine (analogs & derivatives, pharmacology)
• Lymphoma, Non-Hodgkin (enzymology)
• Male
• Mice
• Mice, Inbred CBA
• Tumor Necrosis Factor-alpha (pharmacology)