A MAGE-1 antigenic peptide recognized by human cytolytic T lymphocytes on HLA-A2 tumor cells.

Abstract

"Cancer-germline" genes such as those of the MAGE family are expressed in many tumors and in male germline cells, but are silent in normal tissues. They encode shared tumor-specific antigens that have been used in therapeutic vaccination trials of cancer patients. It was previously demonstrated that MAGE-1 peptide KVLEYVIKV was presented by HLA-A 0201 molecules on the surface of a human breast carcinoma cell line, but no human specific CTL had been isolated so far. Here, we have used HLA-A2/MAGE-1 fluorescent multimers to isolate from blood cells three human CTL clones that recognized the MAGE-1 peptide. These clones killed efficiently HLA-A2 tumor cells expressing MAGE-1, whether or not they were treated with IFN-gamma, suggesting that the MAGE-1 antigen is processed efficiently by both the standard proteasome and the immunoproteasome. These results indicate that the MAGE-1.A2 peptide can be used for antitumoral vaccination.

• Authors: Sabrina Ottaviani, Yi Zhang, Thierry Boon, Pierre van der Bruggen
• Journal: Cancer immunology, immunotherapy : CII (Cancer Immunol Immunother) Vol. 54 Issue 12 Pg. 1214-20 (Dec 2005) ISSN: 0340-7004 [Print] Germany
• PMID: 16025263 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• HLA-A2 Antigen
• MAGE-A1 protein (278-286), human
• Neoplasm Proteins
• Peptide Fragments
• Proteasome Endopeptidase Complex

Topics

• Amino Acid Sequence
• Antigen Presentation
• Antigens, Neoplasm (immunology)
• HLA-A2 Antigen (analysis, metabolism)
• Humans
• Molecular Sequence Data
• Neoplasm Proteins (immunology)
• Neoplasms (immunology)
• Peptide Fragments (immunology)
• Proteasome Endopeptidase Complex (metabolism)
• T-Lymphocytes, Cytotoxic (immunology)