Alpha v beta 6 integrin down-regulates the MMP-13 expression in oral squamous cell carcinoma cells.

Abstract

The integrin alphavbeta6, a receptor for fibronectin, vitronectin, tenascin and TGF-beta latency-associated peptide (LAP), is not detectable on normal oral epithelium but is neo-expressed in oral squamous cell carcinomas (OSCC) and epithelial dysplasia. Previously it has been shown that alphavbeta6 integrin can up-regulate MMP-3 and -9 expression in OSCC cells. Using beta6-transfected and control OSCC cells we demonstrate that alphavbeta6 integrin down-regulates MMP-13 expression at both mRNA and protein level. Although expressing less MMP-13, beta6-transfected cells were found to have similar collagenolytic activity as control cells and invade at similar levels through type I collagen. Growth of the tumour cells in organotypic culture and confocal microscopy confirmed low levels of MMP-13 in cells with high alphavbeta6 expression. Furthermore, human squamous cell carcinomas of the tongue with high expression of alphavbeta6 showed lower MMP-13 levels than carcinomas with low levels of alphavbeta6. Our results suggest that alphavbeta6 down-regulates MMP-13 expression in OSCC cells and that MMP-13 is not essential for the degradation of type I collagen by OSCC cells.

• Authors: M Ylipalosaari, G J Thomas, M Nystrom, S Salhimi, J F Marshall, V Huotari, T Tervahartiala, T Sorsa, T Salo
• Journal: Experimental cell research (Exp Cell Res) Vol. 309 Issue 2 Pg. 273-83 (Oct 01 2005) ISSN: 0014-4827 [Print] United States
• PMID: 16024014 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Collagen Type I
• Integrins
• Matrix Metalloproteinase Inhibitors
• Membrane Proteins
• RNA, Messenger
• integrin alphavbeta6
• Collagenases
• MMP13 protein, human
• Matrix Metalloproteinase 13
• Matrix Metalloproteinase 8
• Matrix Metalloproteinase 1

Topics

• Antigens, Neoplasm (physiology)
• Carcinoma, Squamous Cell (enzymology)
• Cell Line, Tumor
• Collagen Type I (antagonists & inhibitors, metabolism)
• Collagenases (biosynthesis, genetics)
• Down-Regulation (physiology)
• Gene Expression Regulation, Neoplastic (physiology)
• Humans
• Integrins (physiology)
• Matrix Metalloproteinase 1 (biosynthesis, genetics)
• Matrix Metalloproteinase 13
• Matrix Metalloproteinase 8 (biosynthesis, genetics)
• Matrix Metalloproteinase Inhibitors
• Membrane Proteins (antagonists & inhibitors, biosynthesis, genetics)
• Mouth Neoplasms (enzymology, metabolism)
• RNA, Messenger (antagonists & inhibitors, metabolism)