Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus.

Abstract

We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.

• Authors: Takumi Abe, Norifumi Miyake, Yasuyuki Nishijima, Ryousuke Fujita, Ken Sahara, Shin-Ichiro Asano, Hisanori Bando
• Journal: Virus research (Virus Res) Vol. 112 Issue 1-2 Pg. 38-41 (Sep 2005) ISSN: 0168-1702 [Print] Netherlands
• PMID: 16022899 (Publication Type: Journal Article)

Chemical References

• Luciferases
• DNA-Directed RNA Polymerases

Topics

• Animals
• Base Sequence
• Caulimovirus (genetics)
• Cells, Cultured
• DNA-Directed RNA Polymerases (genetics, metabolism)
• Gene Expression Regulation, Viral
• Luciferases (genetics, metabolism)
• Molecular Sequence Data
• Nucleopolyhedroviruses (genetics, physiology)
• Promoter Regions, Genetic (genetics, physiology)
• Recombination, Genetic
• Spodoptera (virology)
• Transcription, Genetic