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Determination of hydrazine in biofluids by capillary gas chromatography with nitrogen-sensitive or mass spectrometric detection.

Abstract
Plasma and liver levels of hydrazine were determined at 10, 30, 90 and 270 min in rats given 0.09, 0.27, 0.84 and 2.53 mmol of hydrazine per kg body weight orally by capillary gas chromatography-mass spectrometry of its pentafluorobenzaldehyde adduct (DFBA, m/z 388) using selected ion monitoring with 15N2-labelled hydrazine as the internal standard (adduct, m/z 390). The mean half-life for hydrazine in the plasma was approximately 2 h but varied with dose. Urinary excretion (0-24 h) of hydrazine and its metabolite acetylhydrazine were determined employing nitrogen-phosphorus detection of the adducts utilising a novel internal standard, pentafluorophenylhydrazine, the adduct of which structurally resembles DFBA. The fraction of the original dose excreted as hydrazine (and acetylhydrazine) declined with increasing dose.
AuthorsN E Preece, S Forrow, S Ghatineh, G J Langley, J A Timbrell
JournalJournal of chromatography (J Chromatogr) Vol. 573 Issue 2 Pg. 227-34 (Jan 17 1992) Netherlands
PMID1601955 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Hydrazines
  • hydrazine
  • acetylhydrazine
Topics
  • Animals
  • Chromatography, Gas
  • Gas Chromatography-Mass Spectrometry
  • Hydrazines (blood, metabolism, urine)
  • Liver (chemistry)
  • Male
  • Rats
  • Rats, Inbred Strains

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