Abstract | BACKGROUND: METHODS: RESULTS: The PGE(2) production by IL-1beta-stimulated GFB was significantly upregulated by glycine. The effect of glycine on IL- 1beta-induced cell proliferation and PGE(2) production was concentration- dependent, reached a peak at 3 mM, and declined slowly at higher doses. The synthesis of PGE(2) by human GFB cultured in the absence of glycine was significantly inhibited by IL-10 and partially induced in cells cultured with glycine. Glycine had no effect on TNF-alpha-induced PGE(2) production. The IL-1beta-driven PGE(2) synthesis was blocked by indomethacin, a COX-1/ COX-2 inhibitor, and by COX-2 inhibitor NS-398. The expression of COX-2 protein was slightly induced by glycine, more evidently by IL-1beta, and mostly enhanced by combined IL-1beta with glycine. CONCLUSION:
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Authors | Xiaohui Rausch-Fan, Christian Ulm, Erika Jensen-Jarolim, Andreas Schedle, George Boltz-Nitulescu, Wolf-Dieter Rausch, Michael Matejka |
Journal | Journal of periodontology
(J Periodontol)
Vol. 76
Issue 7
Pg. 1182-8
(Jul 2005)
ISSN: 0022-3492 [Print] United States |
PMID | 16018763
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cyclooxygenase 2 Inhibitors
- Cyclooxygenase Inhibitors
- Interleukin-1
- Membrane Proteins
- Nitrobenzenes
- Sulfonamides
- N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
- Interleukin-10
- Cyclooxygenase 1
- Cyclooxygenase 2
- PTGS1 protein, human
- PTGS2 protein, human
- Prostaglandin-Endoperoxide Synthases
- Dinoprostone
- Glycine
- Indomethacin
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Topics |
- Analysis of Variance
- Cell Proliferation
(drug effects)
- Cells, Cultured
- Cyclooxygenase 1
- Cyclooxygenase 2
- Cyclooxygenase 2 Inhibitors
- Cyclooxygenase Inhibitors
(pharmacology)
- Dinoprostone
(analysis, antagonists & inhibitors, biosynthesis)
- Dose-Response Relationship, Drug
- Fibroblasts
(drug effects, metabolism)
- Gingiva
(cytology, drug effects, metabolism)
- Glycine
(metabolism, pharmacology)
- Humans
- Indomethacin
(pharmacology)
- Interleukin-1
(pharmacology)
- Interleukin-10
(pharmacology)
- Membrane Proteins
- Nitrobenzenes
(pharmacology)
- Periodontal Diseases
(immunology, microbiology)
- Prostaglandin-Endoperoxide Synthases
(biosynthesis)
- Sulfonamides
(pharmacology)
- Up-Regulation
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