The evaluation of 186Re-labeled antibodies using N2S4 chelate in vitro and in vivo using tumor-bearing nude mice.

Abstract

We have recently described a method for radiolabeling monoclonal antibodies, with metallic radionuclides using a new chelating agent N2S3. Using this chelate the monoclonal antibodies Lym-1 and B72.3 were labeled with 186Re and their biological integrity was evaluated in vitro and in vivo. 186Re-labeled antibodies using N2S4 methodology were found to be stable in human serum and retained their immunoreactivity. Intravenous administration of 0.5 mCi 186Re-labeled antibodies resulted in partial or complete regression of tumor tissue in mice.

• Authors: A Najafi, M M Alauddin, A Sosa, G Q Ma, D C Chen, A L Epstein, M E Siegel
• Journal: International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology (Int J Rad Appl Instrum B) Vol. 19 Issue 2 Pg. 205-12 (Feb 1992) ISSN: 0883-2897 [Print] England
• PMID: 1601674 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Ethylenediamines
• Immunotoxins
• Radioisotopes
• Sulfhydryl Compounds
• tetrakis(2-mercaptoethyl)ethylenediamine
• Rhenium

Topics

• Animals
• Antibodies, Monoclonal (therapeutic use)
• Burkitt Lymphoma (immunology, radiotherapy)
• Ethylenediamines (chemistry)
• Evaluation Studies as Topic
• Female
• Humans
• Immunotoxins (therapeutic use)
• Isotope Labeling (methods)
• Mice
• Mice, Nude
• Neoplasm Transplantation
• Neoplasms, Experimental (immunology, radiotherapy)
• Radioimmunotherapy
• Radioisotopes (therapeutic use)
• Rhenium (therapeutic use)
• Sulfhydryl Compounds (chemistry)