[Effects of salvianolic acid B on expressions of TGF-beta1 and its receptors in liver of rats with dimethylnitrosamine-induced hepatic fibrosis].

Abstract

OBJECTIVE:

To study the therapeutic mechanism of salvianolic acid B (SA-B) in treating hepatic fibrosis.

METHODS:

Twenty-four Wistar rats were randomly divided into 3 groups: normal control group, untreated group and SA-B-treated group. Rats in the untreated and SA-B-treated groups were injected intraperitoneally with 0.5% dimethylnitrosamine (DMN) for 4 weeks, daily for 3 days each week at a dose of 10 microg/kg, to induce hepatic fibrosis. Then, rats in the SA-B-treated group were given SA-B orally for another 4 weeks. Hydroxyproline (Hyp) contents in liver tissue of the rats in 3 groups were determined with HCl hydrolysis, and collagen type I, transforming growth factor-beta 1 (TGF-beta1), transforming growth factor-beta receptor type I (TbetaRI) and transforming growth factor-beta receptor type II (TbetaRII) were detected by Western blotting.

RESULTS:

The Hyp content and the expressions of collagen type I, TGF-beta1, TbetaRI and TbetaRII in the liver tissue of rats in the untreated group increased significantly as compared with those in the normal control group, while those in SA-B-treated group decreased significantly as compared with those in the untreated group.

CONCLUSION:

The therapeutic mechanism of SA-B in treating hepatic fibrosis may be related to inhibiting the expressions of TGF-beta1 and its receptors.

• Authors: Xiao-Ning Wang, Yi-Yang Hu, Cheng-Hai Liu, Ping Liu, Da-Yuan Zhu
• Journal: Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine (Zhong Xi Yi Jie He Xue Bao) Vol. 3 Issue 4 Pg. 286-9 (Jul 2005) ISSN: 1672-1977 [Print] China
• PMID: 16009107 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzofurans
• Collagen Type I
• Receptors, Transforming Growth Factor beta
• TGFB1 protein, human
• Tgfb1 protein, rat
• Transforming Growth Factor beta
• Transforming Growth Factor beta1
• salvianolic acid B
• Protein Serine-Threonine Kinases
• Receptor, Transforming Growth Factor-beta Type II
• Dimethylnitrosamine
• Hydroxyproline

Topics

• Animals
• Benzofurans (pharmacology)
• Collagen Type I (analysis)
• Dimethylnitrosamine
• Hydroxyproline (analysis)
• Liver (metabolism)
• Liver Cirrhosis, Experimental (chemically induced, metabolism)
• Male
• Protein Serine-Threonine Kinases
• Random Allocation
• Rats
• Rats, Wistar
• Receptor, Transforming Growth Factor-beta Type II
• Receptors, Transforming Growth Factor beta (biosynthesis, genetics)
• Transforming Growth Factor beta (biosynthesis, genetics)
• Transforming Growth Factor beta1