[Inhibitory effect of endostatin mediated by lipofectin on transplanted ovarian cancer].

Abstract

OBJECTIVE:

To study the inhibitory effect of endostatin mediated by lipofectin on transplanted ovarian cancer in nude mice.

METHODS:

Constructed recombinant vector pVAX1-sEn expressing human endostatin protein was transfected into ovarian cancer cell line 3AO by lipofectin. mRNA of endostatin was detected by RT-PCR. The expression of endostatin in supernatants was detected by enzyme-linked immunosorbent assay (ELISA). The inhibitory effect of pVAX1-sEn on endothelial cell line ECV-204 was detected by methyl thiazolyl tetrazolium (MTT). By use of lipofectin mediated pVAX1-sEn for intratumor injection, the inhibitory effect on growth of ovarian cancer was observed.

RESULTS:

The result of RT-PCR showed there was a specific band at 610 bp. The expression quantity of endostatin in transfected cell supernatant was (201 +/- 8) ng/ml by ELISA. MTT showed pVAX1-sEn transfected cell supernatant could effectively inhibit the growth of ECV-204, the highest inhibitory ratio was 42%. The tumor volumes in pVAX1-sEn treatment group was (0.85 +/- 0.18) cm(3), significantly smaller than that in normal saline control group (1.90 +/- 0.28) cm(3) and pVAX1 control group (1.78 +/- 0.32) cm(3) (P < 0.05). HE stain in tumor tissue showed that there were obvious necrosis cells in the pVAX1-sEn treatment group, but there were flourishly growing tumor cells in pVAX1 and normal saline control groups.

CONCLUSION:

pVAX1-sEn mediated by lipofectin can effectively inhibit the growth of ovarian cancer.

• Authors: Zeng-tao Wei, Xiao-yan Wang, Jian-chun Dong, Chun-hong Ma, Dong Zhao, Yan Zhang, Wen-sheng Sun
• Journal: Zhonghua fu chan ke za zhi (Zhonghua Fu Chan Ke Za Zhi) Vol. 40 Issue 6 Pg. 396-9 (Jun 2005) ISSN: 0529-567X [Print] China
• PMID: 16008891 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Endostatins
• Phosphatidylethanolamines
• 1,2-dielaidoylphosphatidylethanolamine

Topics

• Animals
• Cell Line, Tumor
• Cell Proliferation
• Down-Regulation
• Endostatins (genetics, metabolism)
• Female
• Gene Expression
• Humans
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Neoplasm Transplantation
• Ovarian Neoplasms (genetics, metabolism, physiopathology)
• Phosphatidylethanolamines
• Random Allocation
• Transfection