Abstract |
Conventional central chondrosarcomas are malignant cartilaginous tumours, occasionally arising secondary to either solitary or multiple ( Ollier disease) enchondromas. Recurrences may have progressed in grade. The aims of the present study were to identify putative differences in gene expression between solitary and Ollier disease-related tumours, and to elucidate signalling pathways involved in tumour progression by genome-wide cDNA expression analysis. Arrays enriched for cartilage-specific cDNAs and genes involved in general tumourigenesis were used to analyse enchondromas (n = 3, two with Ollier disease), chondrosarcomas of different grades (n = 19, three with Ollier disease), normal resting-zone cartilage (n = 2), and chondrosarcoma cells in culture (n = 7). The arrays were analysed by unsupervised hierarchical clustering, significant analysis of microarray, and T-tests. Confirmation of data was performed by immunohistochemistry and quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Ollier disease cases and solitary tumours revealed similar expression profiles, suggesting that the same signalling pathways are involved in tumourigenesis. Interestingly, JunB protein expression was significantly higher in grade I chondrosarcomas than in enchondromas (p = 0.009), which could be of diagnostic relevance. Upon chondrosarcoma progression, matrix-associated genes are down-regulated, reflecting the histology of high-grade tumours. An increase in glycolysis-associated, and a decrease in oxidative phosphorylation-related, genes was found in high-grade tumours. These findings suggest an adaptation in energy supply upon progression towards higher grade.
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Authors | Leida B Rozeman, Liesbeth Hameetman, Tom van Wezel, Antonie H M Taminiau, Anne Marie Cleton-Jansen, Pancras C W Hogendoorn, Judith V M G Bovée |
Journal | The Journal of pathology
(J Pathol)
Vol. 207
Issue 1
Pg. 61-71
(Sep 2005)
ISSN: 0022-3417 [Print] England |
PMID | 16007578
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright (c) 2005 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. |
Chemical References |
- Biomarkers, Tumor
- DNA, Complementary
- DNA, Neoplasm
- Neoplasm Proteins
- Proto-Oncogene Proteins c-jun
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Topics |
- Adolescent
- Adult
- Aged
- Biomarkers, Tumor
(metabolism)
- Bone Neoplasms
(genetics, metabolism, pathology)
- Child
- Chondrosarcoma
(genetics, metabolism, pathology)
- DNA, Complementary
(genetics)
- DNA, Neoplasm
(genetics)
- Disease Progression
- Enchondromatosis
(genetics, metabolism, pathology)
- Female
- Gene Expression Profiling
(methods)
- Gene Expression Regulation, Neoplastic
- Humans
- Infant
- Male
- Middle Aged
- Neoplasm Proteins
(metabolism)
- Oligonucleotide Array Sequence Analysis
(methods)
- Phosphorylation
- Polymerase Chain Reaction
(methods)
- Proto-Oncogene Proteins c-jun
(metabolism)
- Signal Transduction
- Tumor Cells, Cultured
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