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Clinical use of 16S rRNA gene sequencing to identify Mycoplasma felis and M. gateae associated with feline ulcerative keratitis.

Abstract
Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as Mycoplasma felis (six cases) and Mycoplasma gateae (one case). Identification of the pathogens allowed the use of less empirical and more organism-specific therapy.
AuthorsLarry D Gray, Kerry L Ketring, Yi-Wei Tang
JournalJournal of clinical microbiology (J Clin Microbiol) Vol. 43 Issue 7 Pg. 3431-4 (Jul 2005) ISSN: 0095-1137 [Print] United States
PMID16000470 (Publication Type: Case Reports, Journal Article)
Chemical References
  • Culture Media
  • DNA, Bacterial
  • RNA, Ribosomal, 16S
Topics
  • Animals
  • Bacterial Typing Techniques
  • Cat Diseases (diagnosis, microbiology)
  • Cats
  • Cornea (microbiology)
  • Corneal Ulcer (diagnosis, microbiology, veterinary)
  • Culture Media
  • DNA, Bacterial (analysis)
  • Genes, rRNA
  • Molecular Sequence Data
  • Mycoplasma (classification, genetics, isolation & purification)
  • Mycoplasma Infections (diagnosis, microbiology, veterinary)
  • RNA, Ribosomal, 16S (genetics)
  • Sequence Analysis, DNA

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