Abstract |
In order to develop a rat model that reflects human weakly or nonimmunogenic tumor-host relationships and allows investigation of T-cell retargeting with bispecific monoclonal antibodies in vivo, we prepared several mixed hybridomas. One fusion partner was the anti-rat- T-cell receptor (TCR)-framework hybridoma R73 and the others were hybridomas producing antibodies against CC531, a Wag rat colon carcinoma. Stimulation of Wag rat spleen cells with immobilized R73 mAb and rIL-2 yielded predominantly CD8 positive effector T-lymphocytes, which lysed control P815 target cells efficiently in R73-mediated reverse antibody-dependent cellular cytotoxicity (ADCC). The capacity of these effectors to cause significant hybrid antibody-mediated lysis of CC531 emerged several days later, was critically dependent on prolonged stimulation with immobilized R73, and was associated with increased N-alfa-benzyloxycarbonyl- L-lysine thiobenzyl esterase content.
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Authors | G D Beun, D H van Eendenburg, W E Corver, C J van de Velde, G J Fleuren |
Journal | Journal of immunotherapy : official journal of the Society for Biological Therapy
(J Immunother (1991))
Vol. 11
Issue 4
Pg. 238-48
(May 1992)
ISSN: 1053-8550 [Print] United States |
PMID | 1599909
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Antilymphocyte Serum
- CD8 Antigens
- Receptors, Antigen, T-Cell
- Receptors, Interleukin-2
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Topics |
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Antilymphocyte Serum
- CD8 Antigens
- Colonic Neoplasms
(immunology)
- Cytotoxicity, Immunologic
- Female
- In Vitro Techniques
- Lymphocyte Activation
- Male
- Rats
- Receptors, Antigen, T-Cell
- Receptors, Interleukin-2
- T-Lymphocytes
(immunology)
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