Inhibitory effects of sasanquasaponin on over-expression of ICAM-1 and on enhancement of capillary permeability induced by burns in rats.

The purpose of this study was to investigate the effects of sasanquasaponin (SQS) on ICAM-1 expression and capillary permeability induced by burns in rats. Male adult Sprague-Dawley (SD) rats were subjected to burns in the presence or absence of SQS, and then intravenously injected with Evans blue (60.0 mg/kg body weight). The levels of soluble ICAM-1 (sICAM-1) in sera were assayed using ELISA and the expression levels of transmembrane ICAM-1 (mICAM-1) in aorta were determined by Western blots and ICAM-1 mRNA levels were measured using semi-quantification RT-PCR. The capillary permeability was determined spectrophotometrically. The results showed that SQS markedly lowered the levels of sICAM-1 in sera, and considerably inhibited the over-expression as well as transcription of mICAM-1 in rat aorta. In addition, SQS dramatically inhibited the enhancement of dermal capillary permeability induced by burns in a dose-dependent manner. These results suggest that SQS, developed from Chinese traditional herbs, might be effective in decreasing inflammation induced by burns.
AuthorsQiren Huang, Lijian Shao, Ming He, Heping Chen, Dan Liu, Yongming Luo, Yucheng Dai
JournalBurns : journal of the International Society for Burn Injuries (Burns) Vol. 31 Issue 5 Pg. 637-42 (Aug 2005) ISSN: 0305-4179 [Print] England
PMID15993308 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • 22-O-angeloylcamelliagenin C-3-O-(glucopyranosyl-1-2)(glucopyranosyl-1-2-O-arabinopyranosyl-1-3-)glucopyranosiduronic acid
  • Saponins
  • Intercellular Adhesion Molecule-1
  • Animals
  • Aorta (metabolism)
  • Blotting, Western
  • Burns (metabolism)
  • Capillary Permeability (drug effects)
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Intercellular Adhesion Molecule-1 (metabolism)
  • Male
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Saponins (pharmacology)

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