Abstract |
The purpose of this study was to investigate the effects of sasanquasaponin (SQS) on ICAM-1 expression and capillary permeability induced by burns in rats. Male adult Sprague-Dawley (SD) rats were subjected to burns in the presence or absence of SQS, and then intravenously injected with Evans blue (60.0 mg/kg body weight). The levels of soluble ICAM-1 (sICAM-1) in sera were assayed using ELISA and the expression levels of transmembrane ICAM-1 (mICAM-1) in aorta were determined by Western blots and ICAM-1 mRNA levels were measured using semi-quantification RT-PCR. The capillary permeability was determined spectrophotometrically. The results showed that SQS markedly lowered the levels of sICAM-1 in sera, and considerably inhibited the over-expression as well as transcription of mICAM-1 in rat aorta. In addition, SQS dramatically inhibited the enhancement of dermal capillary permeability induced by burns in a dose-dependent manner. These results suggest that SQS, developed from Chinese traditional herbs, might be effective in decreasing inflammation induced by burns.
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Authors | Qiren Huang, Lijian Shao, Ming He, Heping Chen, Dan Liu, Yongming Luo, Yucheng Dai |
Journal | Burns : journal of the International Society for Burn Injuries
(Burns)
Vol. 31
Issue 5
Pg. 637-42
(Aug 2005)
ISSN: 0305-4179 [Print] Netherlands |
PMID | 15993308
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 22-O-angeloylcamelliagenin C-3-O-(glucopyranosyl-1-2)(glucopyranosyl-1-2-O-arabinopyranosyl-1-3-)glucopyranosiduronic acid
- Saponins
- Intercellular Adhesion Molecule-1
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Topics |
- Animals
- Aorta
(metabolism)
- Blotting, Western
- Burns
(metabolism)
- Capillary Permeability
(drug effects)
- Dose-Response Relationship, Drug
- Enzyme-Linked Immunosorbent Assay
- Intercellular Adhesion Molecule-1
(metabolism)
- Male
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Saponins
(pharmacology)
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