A novel function of transcription factor alpha-Pal/NRF-1: increasing neurite outgrowth.

Abstract

Alpha-Pal/NRF-1 is a critical regulator of the promoter of human IAP/CD47 gene, a gene related to memory formation in rodents. However, its function in neurons was unknown. We found that stable or transient expression of full-length alpha-Pal/NRF-1 in human neuroblastoma IMR-32 cells significantly induced neurite outgrowth and increased the length of neurites both in medium containing 10% fetal bovine serum and in serum-free medium. In contrast, the dominant-negative mutant of alpha-Pal/NRF-1 inhibited the induction and extension of neurites. Ectopic expression of full-length alpha-Pal/NRF-1 also increased the induction of neurite outgrowth in primary mouse cortical neurons. The IAP antisense cDNA significantly inhibited the increase of neurite outgrowth by alpha-Pal/NRF-1. These findings indicate that a novel function of alpha-Pal/NRF-1 is to regulate neuronal differentiation, and that this function is mediated partly via its downstream IAP gene.

• Authors: Wen-Teng Chang, Hsiun-ing Chen, Rong-Jing Chiou, Chen-Yun Chen, A-Min Huang
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 334 Issue 1 Pg. 199-206 (Aug 19 2005) ISSN: 0006-291X [Print] United States
• PMID: 15992771 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA-Binding Proteins
• Neoplasm Proteins
• Nuclear Respiratory Factor 1
• Nuclear Respiratory Factors
• Recombinant Proteins
• Trans-Activators
• Transcription Factors
• alpha-Pal transcription factor
• immunosuppressive acidic protein

Topics

• Cell Differentiation
• Cell Line, Tumor
• Cell Proliferation
• DNA-Binding Proteins (genetics, metabolism)
• Humans
• Neoplasm Proteins (metabolism)
• Neurites (metabolism, pathology)
• Neuroblastoma (metabolism, pathology)
• Nuclear Respiratory Factor 1
• Nuclear Respiratory Factors
• Recombinant Proteins (metabolism)
• Trans-Activators (genetics, metabolism)
• Transcription Factors (genetics, metabolism)