| Abstract | Female patients experience substantial neuroprotection after experimental stroke compared with male patients, a finding attributed to the protective effects of gonadal hormones. This study examined the response of male- and female-derived organotypic hippocampal slices to oxidative and excitotoxic injury. Both oxygen and glucose deprivation and N-methyl-D-aspartic acid exposure led to neuronal death; however, female-derived cultures sustained less injury than male-derived cultures. Cell death after oxygen and glucose deprivation was ameliorated in male cultures, but not female cultures, by the addition of 7-nitroindazole, a neuronal nitric oxide synthase inhibitor. These studies have relevance to researchers investigating neuroprotective agents in mixed sex experiments. |
| Authors | Hong Li, Scott Pin, Zhiyuan Zeng, Michael M Wang, Katrin A Andreasson, Louise D McCullough
(Affiliation: Department of Neuropathology, Johns Hopkins School of Medicine, Baltimore, MD, USA.)
|
| Journal | Annals of neurology
(Ann Neurol)
Vol. 58
Issue 2
Pg. 317-21
(Aug 2005)
ISSN: 0364-5134 United States |
| PMID | 15988750
(Publication Type: Comparative Study, In Vitro, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
| Chemical References |
- DNA-Binding Proteins
- High Mobility Group Proteins
- Indazoles
- Neuroprotective Agents
- RNA, Messenger
- SOXB1 Transcription Factors
- Transcription Factors
- Nitric Oxide
- 7-nitroindazole
- Glucose
- N-Methylaspartate
- Superoxide Dismutase
|
| Topics |
- Animals
- Animals, Newborn
- Anoxia
- Cell Death
(drug effects, physiology)
- DNA-Binding Proteins
(genetics)
- Disease Models, Animal
- Drug Interactions
- Female
- Genotype
- Glucose
(deficiency)
- High Mobility Group Proteins
(genetics)
- Hippocampus
(pathology)
- Indazoles
(therapeutic use)
- Male
- N-Methylaspartate
(toxicity)
- Neurons
(drug effects, pathology)
- Neuroprotective Agents
(therapeutic use)
- Nitric Oxide
(metabolism)
- Organ Culture Techniques
- RNA, Messenger
(biosynthesis)
- Rats
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- SOXB1 Transcription Factors
- Sex Characteristics
- Stroke
(etiology, pathology, prevention & control)
- Superoxide Dismutase
(metabolism)
- Time Factors
- Transcription Factors
(genetics)
|
