Influence of phospholipid composition on the adjuvanticity and protective efficacy of liposome-encapsulated Leishmania donovani antigens.

Abstract	In this study, we evaluate the effect of phospholipid on the adjuvanicity and protective efficacy of liposome vaccine carriers against visceral leishmaniasis (VL) in a hamster model. Liposomes prepared with distearyol derivative of L-alpha-phosphatidyl choline (DSPC) having liquid crystalline transition temperature (Tc) 54 C were as efficient as dipalmitoyl (DPPC) (Tc 41 C) and dimyristoyl (DMPC) (Tc 23 C) derivatives in their ability to entrap Leishmania donovani membrane antigens (LAg) and to potentiate strong antigen-specific antibody responses. However, whereas LAg in DPPC and DMPC liposomes stimulated inconsistent delayed type hypersensitivity (DTH) responses, strong DTH was observed with LAg in DSPC liposomes. The heightened adjuvant activity of DSPC liposomes corresponded with 95% protection, with almost no protectivity with LAg in DPPC and DMPC liposomes, 4 mo after challenge with L. donovani. These data demonstrate the superiority of DSPC liposomes for formulation of L. donovani vaccine. In addition, they demonstrate a correlation of humoral and cell-mediated immunity with protection against VL in hamsters.
Authors	Tuhina Mazumdar, K Anam, N Ali
Journal	The Journal of parasitology (J Parasitol) Vol. 91 Issue 2 Pg. 269-74 (Apr 2005) ISSN: 0022-3395 [Print] United States
PMID	15986599 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Adjuvants, Immunologic Antibodies, Protozoan Antigens, Protozoan Liposomes Phospholipids Protozoan Vaccines
Topics	Adjuvants, Immunologic (administration & dosage) Animals Antibodies, Protozoan (biosynthesis, blood) Antigens, Protozoan (administration & dosage, immunology) Cricetinae Disease Models, Animal Enzyme-Linked Immunosorbent Assay Humans Hypersensitivity, Delayed (immunology) Kinetics Leishmania donovani (immunology) Leishmaniasis, Visceral (immunology, prevention & control) Liposomes (chemistry) Mesocricetus Phospholipids (chemistry) Protozoan Vaccines (administration & dosage, immunology) Spleen (parasitology)

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