Abstract |
We investigated the expression pattern of the breast cancer associated gene LIV-1 on mRNA and protein level in 111 human breast cancer patients by in situ hybridization as well as immunohistochemistry and focused on the unknown potential of LIV-1 expression levels as a prognostic marker. To our knowledge, this is the first study on endogenous LIV-1 protein expression. Results of our study indicate that LIV-1 mRNA and protein expression levels are only weakly correlated, suggesting posttranscriptional regulatory mechanisms. Furthermore, LIV-1 mRNA quantity in combination with a positive ER status seem to represent a better marker than the progesterone receptor status according to the prognostic significance for relapse free survival (RFS). A negative correlation of LIV-1 protein levels with tumor size, grade and stage reflects an association of LIV-1 protein expression with less aggressive tumors. High LIV-1 protein expression seems to be associated with a longer relapse free and overall survival in breast cancer patients with invasive ductal carcinoma. This association, however, seems to be dependent from other prognostic markers. Our data suggest that LIV-1 is a promising candidate for a novel marker for breast cancer patients with better outcome. Furthermore, our study presents a revised cDNA sequence of LIV-1 and demonstrates the localization of endogenous LIV-1 in the endoplasmic reticulum.
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Authors | Grit Kasper, Armin A Weiser, Andreas Rump, Katrin Sparbier, Edgar Dahl, Arndt Hartmann, Peter Wild, Uta Schwidetzky, Esmeralda Castaños-Vélez, Kerstin Lehmann |
Journal | International journal of cancer
(Int J Cancer)
Vol. 117
Issue 6
Pg. 961-73
(Dec 20 2005)
ISSN: 0020-7136 [Print] United States |
PMID | 15986450
(Publication Type: Journal Article)
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Copyright | Copyright 2005 Wiley-Liss, Inc |
Chemical References |
- Cation Transport Proteins
- DNA, Complementary
- Neoplasm Proteins
- RNA, Messenger
- Receptors, Estrogen
- SLC39A6 protein, human
- Tamoxifen
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Topics |
- Base Sequence
- Breast Neoplasms
(chemistry, genetics, pathology)
- Cation Transport Proteins
(analysis, genetics)
- DNA, Complementary
(chemistry)
- Disease-Free Survival
- Drug Resistance, Neoplasm
- Endoplasmic Reticulum
(chemistry)
- Gene Expression
- Humans
- Immunohistochemistry
- In Situ Hybridization
- Middle Aged
- Molecular Sequence Data
- Neoplasm Proteins
(analysis, genetics)
- Neoplasm Recurrence, Local
- Prognosis
- RNA, Messenger
(analysis)
- Receptors, Estrogen
(analysis)
- Survival Rate
- Tamoxifen
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