Suppression of generalized seizures activity by intrathalamic 2-chloroadenosine application.

Abstract

In the present study, we investigated the effects of micro-injecting 2-chloroadenosine (2-CADO; an adenosine receptor agonist) into the thalamus alone and with theophylline (a nonspecific adenosine receptor antagonist) pretreatment on pentylenetetrazol (PTZ)-induced tonic-clonic seizures in male Wistar albino rats. Following intrathalamic 2-CADO injection alone or theophylline pretreatment, 50 mg kg(-1) PTZ was given ip after 1 and 24 hrs. The duration of epileptic seizure activity was recorded by cortical electroencephalogram (EEG), and seizure severity was behaviorally scored. Intrathalamic 2-CADO administration induced significant decreases in both seizure duration and seizure severity scores at 1 and 24 hrs, but the effects were more abundant on the seizures induced after 24 hrs. On the other hand, pretreatment with theophylline prevented the inhibitor effect of 2-CADO on seizure activity and increased both seizure duration and seizure scores. Present results suggest that the activation of adenosine receptors in the thalamus may represent another anticonvulsant/modulatory site of adenosine action during the course of the PTZ-induced generalized tonic-clonic seizures and provide additional data for the involvement of the adenosinergic system in the generalized seizures model.

• Authors: Nurbay Ates, Gul Ilbay, Deniz Sahin
• Journal: Experimental biology and medicine (Maywood, N.J.) (Exp Biol Med (Maywood)) Vol. 230 Issue 7 Pg. 501-5 (Jul 2005) ISSN: 1535-3702 [Print] England
• PMID: 15985626 (Publication Type: Journal Article)

Chemical References

• Anticonvulsants
• Purinergic P1 Receptor Agonists
• Purinergic P1 Receptor Antagonists
• 2-Chloroadenosine
• Theophylline
• Pentylenetetrazole

Topics

• 2-Chloroadenosine (administration & dosage, pharmacology, therapeutic use)
• Animals
• Anticonvulsants (administration & dosage, therapeutic use)
• Electroencephalography
• Male
• Pentylenetetrazole (pharmacology)
• Purinergic P1 Receptor Agonists
• Purinergic P1 Receptor Antagonists
• Rats
• Rats, Wistar
• Seizures (chemically induced, prevention & control)
• Thalamus (drug effects, physiology)
• Theophylline (pharmacology)