Histatin and lactoferrin derived peptides: antimicrobial properties and effects on mammalian cells.

Abstract

In order to analyze the clinical potential of two antimicrobial peptides, human lactoferrin 1-11 (hLF1-11) and synthetic histatin analogue Dhvar-5, we measured the killing effect on bacteria, and the potential toxicity on erythrocytes and bone cells. The antimicrobial activity was determined in a killing assay on six strains, including methicillin resistant Staphylococcus Aureus. The effect on human erythrocytes and MC3T3 mouse bone cells was measured with a hemolysis assay and a viability assay, respectively. Both hLF1-11 and Dhvar-5 dose-dependently killed all bacterial strains, starting at concentrations of 6 microg/mL. hLF1-11 had no effect on mammalian cells at concentrations up to 400 microg/mL, but Dhvar-5 induced significant hemolysis (37% at 200 microg/mL) and bone cell death (70% at 400 microg/mL). This indicates that both peptides are able to kill various resistant and non-resistant bacteria, but Dhvar-5 may exert a cytotoxic effect on host cells at higher concentrations.

• Authors: Hein P Stallmann, Chris Faber, Antonius L J J Bronckers, Jolanda M A de Blieck-Hogervorst, Carlo P J M Brouwer, Arie V Nieuw Amerongen, Paul I J M Wuisman
• Journal: Peptides (Peptides) Vol. 26 Issue 12 Pg. 2355-9 (Dec 2005) ISSN: 0196-9781 [Print] United States
• PMID: 15979203 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Infective Agents
• Dhvar-5
• Histatins
• Peptide Fragments
• Salivary Proteins and Peptides
• lactoferrin (1-11), human
• Lactoferrin

Topics

• Animals
• Anti-Infective Agents (pharmacology)
• Bone Marrow Cells (metabolism)
• Cell Death (drug effects)
• Cell Line
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Erythrocytes (drug effects)
• Hemolysis (drug effects)
• Histatins
• Humans
• Lactoferrin
• Methicillin Resistance
• Mice
• Microbial Sensitivity Tests
• Peptide Fragments (pharmacology)
• Salivary Proteins and Peptides (pharmacology)
• Staphylococcus aureus (growth & development)