Dietary phosphatidylcholine alleviates fatty liver induced by orotic acid.

Abstract

OBJECTIVE:

We compared the effect of dietary phosphatidylcholine (PC) with that of triacylglycerol (TG), both with the same fatty acid profiles, on fatty infiltration in orotic acid (OA)-induced fatty liver of Sprague-Dawley rats.

METHODS:

Rats were fed an OA-supplemented diets containing TG (TG+OA group) or PC (20% of dietary lipid, PC+OA group) for 10 d. Rats fed the TG diet without OA supplementation served as the basal group.

RESULTS:

Administering OA significantly increased the weights and TG accumulation in livers of the TG+OA group compared with the basal group. These changes were attributed to significant increases in the activities of fatty acid synthase, malic enzyme, and glucose-6-phosphate dehydrogenase, which are fatty acid synthetic enzymes, and phosphatidate phosphohydrolase, a rate-limiting enzyme of TG synthesis. However, the PC+OA group did not show TG accumulation and OA-induced increases of these enzyme activities. Further, a significant increase in the activity of carnitine palmitoyl transferase, a rate-limiting enzyme of fatty acid beta-oxidation, was found in the PC+OA group.

CONCLUSIONS:

Dietary PC appears to alleviate the OA-induced hepatic steatosis and hepatomegaly, mainly through the attenuation of hepatic TG synthesis and enhancement of fatty acid beta-oxidation in Sprague-Dawley rats.

• Authors: Yohanes Buang, Yu-Ming Wang, Jae-Young Cha, Koji Nagao, Teruyoshi Yanagita
• Journal: Nutrition (Burbank, Los Angeles County, Calif.) (Nutrition) 2005 Jul-Aug Vol. 21 Issue 7-8 Pg. 867-73 ISSN: 0899-9007 [Print] United States
• PMID: 15975496 (Publication Type: Journal Article)

Chemical References

• Phosphatidylcholines
• Phospholipids
• RNA, Messenger
• Triglycerides
• Orotic Acid
• Cholesterol
• Carnitine O-Palmitoyltransferase
• Fatty Acid Synthases

Topics

• Animals
• Carnitine O-Palmitoyltransferase (genetics, metabolism)
• Cholesterol (blood)
• Fatty Acid Synthases (genetics, metabolism)
• Fatty Liver (chemically induced, drug therapy, enzymology)
• Liver (chemistry, drug effects, enzymology)
• Male
• Organ Size (drug effects)
• Orotic Acid (toxicity)
• Oxidation-Reduction
• Phosphatidylcholines (administration & dosage, metabolism, therapeutic use)
• Phospholipids (blood, metabolism)
• RNA, Messenger (metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Triglycerides (administration & dosage, blood, metabolism)