Immunoreactivity of a mammalian liver component with leprosy sera.

Abstract

Sera from 77 leprosy patients in various stages of infection--tuberculoid (TT), lepromatous (LL), borderline tuberculoid and borderline lepromatous--15 contacts and 21 normal healthy individuals, were assayed in an indirect enzyme-linked immunosorbent assay and dot enzyme immunoassay using ethanol-soluble and thermostable extract of liver as the antigen. The highest incidences of reaction were found in untreated LL patients (100%) and in TT patients (91%), while the sera from borderline patients showed a comparatively lower incidence (43%). Some of the sera from contacts of leprosy patients (6/15) also showed high reactivity. Assays using lecithin as an antigen did not exhibit any reaction.

• Authors: R G Deshpande, M B Khan, R G Navalkar
• Journal: International archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 97 Issue 4 Pg. 345-9 ( 1992) ISSN: 1018-2438 [Print] Switzerland
• PMID: 1597354 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Phosphatidylcholines

Topics

• Animals
• Electrophoresis, Polyacrylamide Gel
• Enzyme-Linked Immunosorbent Assay
• Humans
• Immunoblotting
• Immunodiffusion
• Leprosy (immunology)
• Liver (immunology)
• Phosphatidylcholines (immunology)
• Rabbits
• Rats