Induction method of tyrosine kinase A-mediated cell death in rat pheochromocytoma.

Abstract

Nerve Growth Factor (NGF) is a neurotrophic factor that prevents apoptosis in neuronal progenitor cells. In rat pheochromocytoma (PC12) cells, tyrosine kinase A receptor (TrkA) mediates neurotrophic or protective effects, while p75 neurotrophin receptor (p75NTR) functions as a death receptor. We have determined whether TrkA mediates any cytotoxic effect. Following serum deprivation, TrkA expression increased 2.2-fold and apoptosis began with expression of Bax proapoptotic protein. Application of NGF halved cell viability but this was reversed by K252a, the TrkA inhibitor. These results confirmed the paradoxical cytotoxic effect of neurotrophic NGF via TrkA in PC12 cells following serum deprivation.

• Authors: Jin-Young Ahn, Lami Kang, Hyun-Jeong Kim, Hyejin Kang, Sung-Hoon Kim, Je-Yong Kim, Seung Ki Kim, Kyu-Chang Wang, Byung Kyu Cho, Kwang-Woo Lee, Jae-Kyu Roh, Manho Kim
• Journal: Biotechnology letters (Biotechnol Lett) Vol. 27 Issue 8 Pg. 583-7 (Apr 2005) ISSN: 0141-5492 [Print] Netherlands
• PMID: 15973493 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carbazoles
• Culture Media, Serum-Free
• Enzyme Inhibitors
• Indole Alkaloids
• Nerve Growth Factor
• staurosporine aglycone
• Receptor, trkA

Topics

• Animals
• Apoptosis (drug effects)
• Blotting, Western
• Carbazoles (pharmacology)
• Cell Survival (drug effects)
• Culture Media, Serum-Free (pharmacology)
• Enzyme Inhibitors (pharmacology)
• Indole Alkaloids
• Nerve Growth Factor (pharmacology)
• PC12 Cells
• Pheochromocytoma (metabolism, pathology)
• Rats
• Receptor, trkA (antagonists & inhibitors, metabolism)