Therapeutic expression of the platelet-specific integrin, alphaIIbbeta3, in a murine model for Glanzmann thrombasthenia.

Abstract

Integrins mediate the adhesion of cells to each other and to the extracellular matrix during development, immunity, metastasis, thrombosis, and wound healing. Molecular defects in either the alpha- or beta-subunit can disrupt integrin synthesis, assembly, and/or binding to adhesive ligands. This is exemplified by the bleeding disorder, Glanzmann thrombasthenia (GT), where abnormalities of the platelet-specific integrin, alphaIIbbeta3, prevent platelet aggregation following vascular injury. We previously used a retrovirus vector containing a cDNA cassette encoding human integrin beta3 to restore integrin alphaIIbbeta3 on the surface of megakaryocytes derived from peripheral blood stem cells of GT patients. In the present study, bone marrow from beta3-deficient (beta3-/-) mice was transduced with the ITGbeta3-cassette to investigate whether the platelet progeny could establish hemostasis in vivo. A lentivirus transfer vector equipped with the human ITGA2B gene promoter confined transgene expression to the platelet lineage. Human beta3 formed a stable complex with murine alphaIIb, effectively restoring platelet function. Mice expressing significant levels of alphaIIbbeta3 on circulating platelets exhibited improved bleeding times. Intravenous immunoglobulin effectively diminished platelet clearance in animals that developed an antibody response to alphaIIbbeta3. These results indicate the feasibility of targeting platelets with genetic therapies for better management of patients with inherited bleeding disorders.

• Authors: Juan Fang, Kairbaan Hodivala-Dilke, Bryon D Johnson, Lily M Du, Richard O Hynes, Gilbert C White 2nd, David A Wilcox
• Journal: Blood (Blood) Vol. 106 Issue 8 Pg. 2671-9 (Oct 15 2005) ISSN: 0006-4971 [Print] United States
• PMID: 15972454 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Immunoglobulins
• Platelet Glycoprotein GPIIb-IIIa Complex

Topics

• Animals
• Blood Platelets (metabolism)
• Disease Models, Animal
• Genetic Therapy
• Humans
• Immunoglobulins (blood, immunology)
• Mice
• Organ Specificity
• Platelet Aggregation
• Platelet Glycoprotein GPIIb-IIIa Complex (genetics, metabolism, therapeutic use)
• Promoter Regions, Genetic (genetics)
• Thrombasthenia (genetics, metabolism, pathology, therapy)