Abstract |
To study the effect of realgar on expression of survivin in leukemia cell lines, HL-60 and Jurket cell lines were used as in vitro models. The expression of survivin was detected by Western blot analysis and immunofluorescence, and the expressions of Fas and caspase-3 were examined by immunohistochemistry. The results showed that the expression of survivin was positive in the two cell lines. HL-60 cells did not express Fas and caspase-3, and Jurket cells were Fas-positive and caspase-3 was negative. Realgar induced a dose- and time-dependent down-regulation of survivin expression in Jurket cells, and especially in HL-60. Caspase-3 expression changed from negative to positive in HL-60 cell, but there still was no expression in Jurket cell. It is concluded that survivin expression level decreased during leukemia cell apoptosis induced by Realgar. The down-regulation of survivin expression may be an important mechanism in leukemia cell apoptosis induced by realgar through mitochondrial pathway.
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Authors | Yan-Feng Xiao, Ya Liu, Shan-Xi Liu, Li-Fen Ren |
Journal | Zhongguo shi yan xue ye xue za zhi
(Zhongguo Shi Yan Xue Ye Xue Za Zhi)
Vol. 13
Issue 3
Pg. 386-90
(Jun 2005)
ISSN: 1009-2137 [Print] China |
PMID | 15972126
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Arsenicals
- BIRC5 protein, human
- Inhibitor of Apoptosis Proteins
- Microtubule-Associated Proteins
- Neoplasm Proteins
- Sulfides
- Survivin
- fas Receptor
- arsenic disulfide
- Caspase 3
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Arsenicals
(pharmacology)
- Blotting, Western
- Caspase 3
(metabolism)
- Dose-Response Relationship, Drug
- Fluorescent Antibody Technique
- HL-60 Cells
- Humans
- Immunohistochemistry
- Inhibitor of Apoptosis Proteins
- Jurkat Cells
- Leukemia
(metabolism, pathology)
- Microtubule-Associated Proteins
(biosynthesis)
- Neoplasm Proteins
(biosynthesis)
- Sulfides
(pharmacology)
- Survivin
- Time Factors
- fas Receptor
(metabolism)
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