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[Effect of realgar on expression of survivin in leukemia cell lines and its significance].

Abstract
To study the effect of realgar on expression of survivin in leukemia cell lines, HL-60 and Jurket cell lines were used as in vitro models. The expression of survivin was detected by Western blot analysis and immunofluorescence, and the expressions of Fas and caspase-3 were examined by immunohistochemistry. The results showed that the expression of survivin was positive in the two cell lines. HL-60 cells did not express Fas and caspase-3, and Jurket cells were Fas-positive and caspase-3 was negative. Realgar induced a dose- and time-dependent down-regulation of survivin expression in Jurket cells, and especially in HL-60. Caspase-3 expression changed from negative to positive in HL-60 cell, but there still was no expression in Jurket cell. It is concluded that survivin expression level decreased during leukemia cell apoptosis induced by Realgar. The down-regulation of survivin expression may be an important mechanism in leukemia cell apoptosis induced by realgar through mitochondrial pathway.
AuthorsYan-Feng Xiao, Ya Liu, Shan-Xi Liu, Li-Fen Ren
JournalZhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 13 Issue 3 Pg. 386-90 (Jun 2005) ISSN: 1009-2137 [Print] China
PMID15972126 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antineoplastic Agents
  • Arsenicals
  • BIRC5 protein, human
  • Inhibitor of Apoptosis Proteins
  • Microtubule-Associated Proteins
  • Neoplasm Proteins
  • Sulfides
  • Survivin
  • fas Receptor
  • arsenic disulfide
  • Caspase 3
Topics
  • Antineoplastic Agents (pharmacology)
  • Apoptosis (drug effects)
  • Arsenicals (pharmacology)
  • Blotting, Western
  • Caspase 3 (metabolism)
  • Dose-Response Relationship, Drug
  • Fluorescent Antibody Technique
  • HL-60 Cells
  • Humans
  • Immunohistochemistry
  • Inhibitor of Apoptosis Proteins
  • Jurkat Cells
  • Leukemia (metabolism, pathology)
  • Microtubule-Associated Proteins (biosynthesis)
  • Neoplasm Proteins (biosynthesis)
  • Sulfides (pharmacology)
  • Survivin
  • Time Factors
  • fas Receptor (metabolism)

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