[Detection of EWS-WT1 fusion transcripts in paraffin-embedded tissues for desmoplastic small round cell tumor].

Abstract

OBJECTIVE:

To detect the EWS-WT1 chimeric mRNA in desmoplastic small round cell tumor (DSRCT) and its clinicopathological significance.

METHODS:

Formalin fixed, paraffin-embedded tumor specimens from 4 examples of this entity were studied by reverse transcriptase polymerase chain reaction to detect the EWS-WT1 fusion transcripts resulting from the chromosomal translocation t(11;22)(p13;q12). The following tumor specimens were included as controls: 2 Ewing's sarcomas/primitive neuroectodermal tumors (PNET/ES), 2 alveolar rhabdomyosarcomas, and 2 lymphomas.

RESULTS:

EWS-WT1 fusion transcripts were detected in 3 of 4 desmoplastic small round cell tumors but not in any other tumor types studied as controls.

CONCLUSION:

The analysis of the EWS-WT1 fusion transcript which was performed on formalin-fixed, paraffin-embedded tissues is a sensitive and specific method in the diagnosis and differential diagnosis of DSRCT.

• Authors: Yu Yang, Hua Wang, Yu-ping Wang, Wei Zhang, Yun-zhong Hui, Zi-hui Wang, Jie Zheng
• Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (Beijing Da Xue Xue Bao Yi Xue Ban) Vol. 37 Issue 3 Pg. 325-8 (Jun 18 2005) ISSN: 1671-167X [Print] China
• PMID: 15968330 (Publication Type: English Abstract, Journal Article)

Chemical References

• Biomarkers, Tumor
• EWS1-WT1 fusion protein, human
• Oncogene Proteins, Fusion

Topics

• Abdominal Neoplasms (genetics, pathology)
• Adult
• Biomarkers, Tumor (analysis)
• Carcinoma, Small Cell (genetics, pathology)
• Female
• Humans
• Male
• Middle Aged
• Oncogene Proteins, Fusion (analysis)
• Paraffin Embedding