The effects of 1-nitropyrene on oxidative DNA damage and expression of DNA repair enzymes.

Abstract

Nitropyrenes (NPs) present in diesel and gasoline emissions are mutagenic and carcinogenic in experimental animals. Nitro-reduction of 1-NP causes oxidative stress. It is unclear whether 8-hydroxydeoxyguanosine (8-OH-dG) is produced from 1-NP and whether it contributes to the carcinogenic activity of 1-NP. In this study, we measured the level of reactive oxygen species (ROS) in cultured human lung epithelial cells after exposure to 1-NP and the intracellular level of 8-OH-dG and expression level of the 8-OH-dG repair enzymes. As results, 1-NP induced the generation of 8-OH-dG via ROS, but 8-OH-dG repair enzymes prevented an increase of 8-OH-dG formation in cellular DNA of the A549 cell line below 250 microM of 1-NP. These data suggest that 1-NP can induce oxidative DNA damage by generation of ROS, which may play a role in the carcinogenesis induced by 1-NP. These data also suggest that individuals with impaired DNA repair enzymes might be more susceptible to lung cancer induced by 1-NP.

• Authors: Yong-Dae Kim, Young-Jun Ko, Toshihiro Kawamoto, Heon Kim
• Journal: Journal of occupational health (J Occup Health) Vol. 47 Issue 3 Pg. 261-6 (May 2005) ISSN: 1341-9145 [Print] Australia
• PMID: 15953848 (Publication Type: Journal Article)

Chemical References

• Mutagens
• Pyrenes
• 8-Hydroxy-2'-Deoxyguanosine
• DNA Repair Enzymes
• Deoxyguanosine
• 1-nitropyrene

Topics

• 8-Hydroxy-2'-Deoxyguanosine
• Cell Culture Techniques
• Cell Line
• DNA Damage (drug effects)
• DNA Repair Enzymes (metabolism)
• Deoxyguanosine (analogs & derivatives, biosynthesis)
• Humans
• Japan
• Lung Neoplasms (chemically induced)
• Mutagens (toxicity)
• Oxidative Stress (drug effects)
• Pyrenes (toxicity)