Sesquin, a potent defensin-like antimicrobial peptide from ground beans with inhibitory activities toward tumor cells and HIV-1 reverse transcriptase.

Abstract

An antifungal peptide with a molecular mass around 7 kDa and an N-terminal sequence highly homologous to defensin was isolated from ground beans (Vigna sesquipedalis cv. 'Ground Bean'). The peptide was adsorbed on Affi-gel blue gel and on Mono S. It exerted an antifungal action on Botrytis cinerea, Fusarium oxysporum and Mycosphaerella arachidicola; and an antibacterial action on Escherichia coli B, Proteus vulgaris, Mycobacterium phlei and Bacillus megaterium. The antimicrobial activity was inhibited in presence of the 5 mM CaCl2 and MgCl2, but no inhibition was observed in 5 mM NaCl. The peptide exerted antiproliferative activity toward breast cancer (MCF-7) cells and leukemia M1 cells, this activity could not be inhibited by the ions mentioned above. It also exhibited some inhibitory activity toward human immunodeficiency virus-type 1 reverse transcriptase.

• Authors: Jack Ho Wong, Tzi Bun Ng
• Journal: Peptides (Peptides) Vol. 26 Issue 7 Pg. 1120-6 (Jul 2005) ISSN: 0196-9781 [Print] United States
• PMID: 15949629 (Publication Type: Journal Article)

Chemical References

• Antifungal Agents
• Antineoplastic Agents, Phytogenic
• Defensins
• Reverse Transcriptase Inhibitors
• HIV Reverse Transcriptase

Topics

• Animals
• Antifungal Agents (isolation & purification, pharmacology)
• Antineoplastic Agents, Phytogenic (isolation & purification, pharmacology)
• Defensins (isolation & purification, pharmacology)
• Fabaceae (chemistry)
• HIV Reverse Transcriptase (antagonists & inhibitors)
• Humans
• Mice
• Reverse Transcriptase Inhibitors (isolation & purification, pharmacology)
• Tumor Cells, Cultured