Abstract | BACKGROUND: METHODS: Tissue was obtained from radical prostatectomies due to prostate adenocarcinoma. Cells were isolated after sequential enzyme digestion and cultured in defined media. Nerve growth factor ( NGF) receptors in untreated cultures were estimated by immunofluorescence. Cultures were treated with leuprolide (agonist) or cetrorelix (antagonist) and expression of TrkA and p75 receptors were evaluated by semi quantitative RT-PCR (polymerase chain reaction) and western blot. Cell proliferation was estimated by MTT method and apoptosis through COMET assay. RESULTS: Both leuprolide and cetrorelix induced a significant increase in p75 receptor gene and protein expression at a concentration that induce apoptosis and decrease proliferation. TrkA receptors showed no changes in presence of GnRH analogs. CONCLUSIONS:
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Authors | Catherine Sánchez, Marisa Clementi, Dixan Benitez, Héctor Contreras, Christian Huidobro, Enrique Castellón |
Journal | The Prostate
(Prostate)
Vol. 65
Issue 3
Pg. 195-202
(Nov 01 2005)
ISSN: 0270-4137 [Print] United States |
PMID | 15948150
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2005 Wiley-Liss, Inc |
Chemical References |
- Antineoplastic Agents, Hormonal
- Formazans
- Receptor, Nerve Growth Factor
- Receptors, Nerve Growth Factor
- Tetrazolium Salts
- MTT formazan
- Gonadotropin-Releasing Hormone
- Receptor, trkA
- Leuprolide
- cetrorelix
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Topics |
- Adenocarcinoma
(drug therapy, metabolism, pathology)
- Antineoplastic Agents, Hormonal
(pharmacology)
- Apoptosis
(drug effects)
- Blotting, Western
- Cell Proliferation
(drug effects)
- Comet Assay
- Epithelial Cells
(pathology)
- Formazans
(chemistry)
- Gonadotropin-Releasing Hormone
(analogs & derivatives, pharmacology)
- Humans
- Leuprolide
(pharmacology)
- Male
- Prostatic Neoplasms
(drug therapy, metabolism, pathology)
- Receptor, Nerve Growth Factor
- Receptor, trkA
(biosynthesis, genetics, metabolism)
- Receptors, Nerve Growth Factor
(biosynthesis, genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Tetrazolium Salts
(chemistry)
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