[Mechanism of apoptosis induced by specific COX-2 inhibitor SC236 in gastric cancer cells].

Abstract

OBJECTIVE:

To investigate the underlying mechanism of apoptosis-inducing effect of a specific COX-2 inhibitor SC236 in gastric cancer cells.

METHODS:

Western blot analysis was used to measure apoptosis-related proteins, cytochrome c, and caspase-3. The catalytic activity of the caspases was measured using a colorimetric assay.

RESULTS:

Treatment of AGS gastric cancer cells with SC236 caused a significant elevation of the pro-apoptotic protein Bak, release of cytochrome c to the cytosol, and activation of caspase-3. A specific caspase-3 inhibitor, z-DEVD-fmk, blocked SC236-induced apoptosis.

CONCLUSION:

SC236 inhibits cell growth and induces apoptosis in gastric cancer cells at least partly through the up-regulation of Bak, stimulation of cytochrome c release, and activation of caspase-3.

• Authors: Xiao-ming Fan, Fa-shou Zheng, Hong-yan Liu, Yuan-hua Ma, B C Y Wong
• Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (Zhonghua Zhong Liu Za Zhi) Vol. 27 Issue 3 Pg. 145-7 (Mar 2005) ISSN: 0253-3766 [Print] China
• PMID: 15946562 (Publication Type: English Abstract, Journal Article)

Chemical References

• 4-(5-(4-chlorophenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)benzenesulfonamide
• Cyclooxygenase 2 Inhibitors
• Oligopeptides
• Pyrazoles
• Sulfonamides
• bcl-2 Homologous Antagonist-Killer Protein
• benzoylcarbonyl-aspartyl-glutamyl-valyl-aspartyl-fluoromethyl ketone
• Cytochromes c
• Poly(ADP-ribose) Polymerases
• CASP3 protein, human
• Caspase 3
• Caspases

Topics

• Adenocarcinoma (metabolism, pathology)
• Apoptosis (drug effects)
• Caspase 3
• Caspases (metabolism)
• Cell Line, Tumor
• Cyclooxygenase 2 Inhibitors (pharmacology)
• Cytochromes c (metabolism)
• Humans
• Oligopeptides (pharmacology)
• Poly(ADP-ribose) Polymerases (metabolism)
• Pyrazoles (pharmacology)
• Stomach Neoplasms (metabolism, pathology)
• Sulfonamides (pharmacology)
• bcl-2 Homologous Antagonist-Killer Protein (metabolism)