Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression.

Abstract

Jasmonic acid (JA) and its methyl ester, methyl jasmonate (MeJA), are plant lipid derivatives that resemble mammalian eicosanoids in structure and biosynthesis. These compounds are proposed to play a role in plant wound and pathogen responses. Here we report the quantitative determination of JA/MeJA in planta by a procedure based on the use of [13C,2H3]MeJA as an internal standard. Wounded soybean (Glycine max [L] Merr. cv. Williams) stems rapidly accumulated MeJA and JA. Addition of MeJA to soybean suspension cultures also increased mRNA levels for three wound-responsive genes (chalcone synthase, vegetative storage protein, and proline-rich cell wall protein) suggesting a role for MeJA/JA in the mediation of several changes in gene expression associated with the plants' response to wounding.

• Authors: R A Creelman, M L Tierney, J E Mullet
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 89 Issue 11 Pg. 4938-41 (Jun 01 1992) ISSN: 0027-8424 [Print] United States
• PMID: 1594598 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Cyclopentanes
• Oxylipins
• Plant Proteins, Dietary
• RNA, Messenger
• jasmonic acid
• Acyltransferases
• flavanone synthetase

Topics

• Acyltransferases (genetics)
• Cell Wall
• Cyclopentanes (chemistry)
• Gene Expression Regulation
• Mass Spectrometry
• Oxylipins
• Plant Proteins, Dietary (genetics)
• RNA, Messenger (genetics)
• Soybeans (genetics, physiology)
• Wound Healing