Abstract | OBJECTIVE: DESIGN: Clinical methods comparative study. METHODS: Urine samples from patients with primary hyperaldosteronism due to either adenoma (n = 6), BAH (n = 6), GSH (n = 9), or essential hypertension (n = 38) were analysed without knowledge of the diagnosis using three different methods in different laboratories. These included 'in-house' radioimmunoassay (RIA), 'in-house' time-resolved fluorometric assay (DELFIA), and gas chromatography mass spectrometry (GC-MS). RESULTS: The three assays showed good correlation, but there were large bias differences: RIA bias was greater than DELFIA which was greater than GC-MS. Discrimination between adenoma and BAH patients was best for the DELFIA method, with no overlap between results for these two groups. All three methods gave significantly elevated results for the GSH group compared with the BAH and essential hypertension groups. No assay distinguished BAH from essential hypertension. CONCLUSION: Measurement of urine 18-OHF may be a useful additional test in the differential diagnosis of primary hyperaldosteronism. The clinical diagnostic value of urinary 18-OHF measurements is method-dependent with the DELFIA assay having the best discriminatory value.
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Authors | R M Reynolds, L A Shakerdi, K Sandhu, A M Wallace, P J Wood, B R Walker |
Journal | European journal of endocrinology
(Eur J Endocrinol)
Vol. 152
Issue 6
Pg. 903-7
(Jun 2005)
ISSN: 0804-4643 [Print] England |
PMID | 15941931
(Publication Type: Comparative Study, Evaluation Study, Journal Article)
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Chemical References |
- 18-hydroxycortisol
- Hydrocortisone
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Topics |
- Adenoma
(urine)
- Adrenal Hyperplasia, Congenital
(urine)
- Diagnosis, Differential
- Fluorometry
(methods)
- Gas Chromatography-Mass Spectrometry
(methods)
- Humans
- Hydrocortisone
(analogs & derivatives, urine)
- Hyperaldosteronism
(urine)
- Hypertension
(urine)
- Radioimmunoassay
(methods)
- Random Allocation
- Statistics, Nonparametric
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