| Abstract | Parkinson's disease (PD) is a common neurodegenerative disease that exhibits motor dysfunctions, such as tremor, akinesia and rigidity. In the present study, we investigated whether swim-test could be used as one of the behavioural monitoring techniques to study motor disability in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced parkinsonism in two mouse strains, Balb/c and C57BL/6. Mice were treated with different doses of MPTP (10, 20 and 30 mg/kg, twice, 16 h apart), and were subjected to swim-test on the third day of the first MPTP injection. MPTP-induced tremor was monitored at 30 min, and akinesia and rigidity developed were studied 3 h after the second MPTP treatment. While tremor and akinesia produced were dose-dependent and the intensity of tremor was comparable in the two strains of mice studied, the latter response in C57BL/6 was significantly lesser than that observed in Balb/c. Rigidity exhibited in Balb/c mice were dose-dependent, but not in C57BL/6. There was observed an inverse relationship between swim-score and the doses of MPTP in both the strains. MPTP caused a significant and dose-dependent reduction in striatal dopamine level in both the strains of mice, when assayed on the fourth day employing an HPLC with electrochemical detector. A significant positive correlation existed (r = 0.94 for Balb/c and r = 0.82 for C57BL/6) for the striatal dopamine-depletion and the swim-score in the MPTP-treated mice. While swim deficit and striatal dopamine loss were long lasting (till the third week) in C57BL/6, in Balb/c mice the motor deficit showed recovery by the second week. In these animals, a significant attenuation in striatal dopamine loss was observed by the third week. These results indicate that swim ability is directly proportional to striatal dopamine content, and suggest that swim-test could be used as a major technique to monitor motor dysfunction in experimental animals. |
| Authors | Reena Haobam, Kizhakke M Sindhu, Goutam Chandra, Kochupurackal P Mohanakumar
(Affiliation: Division of Clinical & Experimental Neuroscience, Indian Institute of Chemical Biology, Calcutta, India.)
|
| Journal | Behavioural brain research
(Behav Brain Res)
Vol. 163
Issue 2
Pg. 159-67
(Sep 8 2005)
ISSN: 0166-4328 Netherlands |
| PMID | 15941598
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Dopamine Agents
- 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
- Serotonin
- Dopamine
|
| Topics |
- 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
(administration & dosage)
- Animals
- Behavior, Animal
- Corpus Striatum
(drug effects, metabolism)
- Disease Models, Animal
- Dopamine
(metabolism)
- Dopamine Agents
(administration & dosage)
- Dose-Response Relationship, Drug
- Freezing Reaction, Cataleptic
(drug effects, physiology)
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Motor Activity
(drug effects)
- Motor Neuron Disease
(etiology, metabolism)
- Movement Disorders
(etiology)
- Parkinson Disease
(physiopathology)
- Parkinsonian Disorders
(metabolism, physiopathology)
- Serotonin
(metabolism)
- Species Specificity
- Statistics as Topic
- Swimming
- Tremor
(etiology)
|
