Abstract |
We analyzed chromosome aberrations, micronucleus frequency, mitotic index (MI), and nuclear division index (NDI) in peripheral lymphocytes of sheep subchronically exposed to the fungicide Euparen Multi (containing 50% tolylfluanid). Euparen Multi was administered by rumen sonde to group of Merino sheep (seven sheep/group) at 93 mg/kg body weight (1/20 LD50) daily for 28 days to assess its genotoxic effects. The frequencies of aberrant cells (ABC) in the experimental and control groups were 5.50+/-1.38% and 2.40+/-1.14%, respectively, and the increase in ABC in the treated group was significant (P = 0.003). Significantly increased numbers of chromatid breaks (5.67+/-1.21% against 2.40+/-1.14%; P = 0.001), chromatid gaps (10.33+/-2.73% against 4.00+/-1.23%; P = 0.001), and chromosome gaps (1.83+/-0.75% against 0.80+/-0.45%; P = 0.025) and exchanges (3.17+/-1.94% against 0.20+/-0.45%; P = 0.009) were observed in exposed animals in comparison to control animals. The frequency of micronuclei (MN) was 29.40+/-5.86 per 1000 binucleated cells in peripheral lymphocytes of sheep in the control group and 49.57+/-19.12 per 1000 binucleated cells in the treated group. A significant increase in the frequency of MN in peripheral lymphocytes also was observed between the two groups (P = 0.0477). No statistical differences in MI and NDI values were found in the groups (P = 0.181 and 0.761, respectively). Thus, our results suggest that exposure to Euparen Multi may cause genome damage in somatic cells.
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Authors | Irena Sutiaková, Natália Kovalkovicová, Juraj Pistl, Jaroslav Novotný, Jaroslav Legáth, Gabriel Kovác, Sabina Hlincíková, Václav Sutiak |
Journal | Ecotoxicology and environmental safety
(Ecotoxicol Environ Saf)
Vol. 64
Issue 3
Pg. 312-20
(Jul 2006)
ISSN: 0147-6513 [Print] Netherlands |
PMID | 15941588
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Aniline Compounds
- Antifungal Agents
- Mutagens
- Sulfonamides
- Toluidines
- N-dichlorofluoromethylthio-N',N'-dimethyl-N-p-tolylsulfamide
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Topics |
- Aniline Compounds
(toxicity)
- Animals
- Antifungal Agents
(toxicity)
- Chromosome Aberrations
(chemically induced)
- Female
- Karyotyping
- Lymphocytes
(drug effects, pathology)
- Micronuclei, Chromosome-Defective
(chemically induced)
- Micronucleus Tests
- Mitotic Index
- Mutagens
(toxicity)
- Sheep
(genetics)
- Sulfonamides
(toxicity)
- Toluidines
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