Selectivity and sensitivity in the detection of single nucleotide polymorphisms (SNPs) are among most important attributes to determine the performance of
DNA microarrays. We previously reported the generation of a novel mesospaced surface prepared by applying
dendron molecules on the solid surface.
DNA microarrays that were fabricated on the
dendron-modified surface exhibited outstanding performance for the detection of single
nucleotide variation in the synthetic
oligonucleotide DNA.
DNA microarrays on the
dendron-modified surface were subjected to the detection of single
nucleotide variations in the exons 5-8 of the p53 gene in genomic DNAs from
cancer cell lines.
DNA microarrays on the
dendron-modified surface clearly discriminated single
nucleotide variations in hotspot
codons with high selectivity and sensitivity. The ratio between the fluorescence intensity of perfectly matched duplexes and that of single
nucleotide mismatched duplexes was >5-100 without sacrificing signal intensity. Our results showed that the outstanding performance of
DNA microarrays fabricated on the
dendron-modified surface is strongly related to novel properties of the
dendron molecule, which has the conical structure allowing mesospacing between the capture probes. Our microarrays on the
dendron-modified surface can reduce the steric hindrance not only between the solid surface and target
DNA, but also among immobilized capture probes enabling the hybridization process on the surface to be very effective. Our
DNA microarrays on the
dendron-modified surface could be applied to various analyses that require accurate detection of SNPs.