| Abstract | To develop a multiantigenic vaccine against toxoplasmosis, two Toxoplasma gondii antigens, SAG1 and GRA4 selected on the basis of previous immunological and immunization studies, were chosen. We showed that DNA-based immunization with plasmids expressing GRA4 (pGRA4) or SAG1 (pSAG1mut) reduced mortality of susceptible C57BL/6 mice upon oral challenge with cysts of the 76K type II strain (62% survival). Immunization with pGRA4 and pSAG1mut, enhanced the protection (75% survival). This protection was further increased by co-inoculation with a plasmid encoding the granulocyte-macrophage colony-stimulating factor (GM-CSF) (87% survival). This latter DNA cocktail provided significant protection of less susceptible outbred Swiss OF1 mice against the development of cerebral cysts. A significantly higher survival of newborns from immunized outbred mice exposed to infection during gestation was observed (4.25+/-3.77 live pups/litter) in comparison to non-immunized mice (1.08+/-2.15 live pups/litter) without preventing parasite vertical transmission. Analysis of the immune response showed that protected animals developed a specific humoral and cellular Th1 response to native T. gondii SAG1 and GRA4 antigens. Our data demonstrated that protection was improved by associating antigens (SAG1 and GRA4) and cytokine (GM-CSF) for further development of a multiantigenic vaccine against toxoplasmosis. |
| Authors | Marie-Noëlle Mévélec, Daniel Bout, Benoît Desolme, Hervé Marchand, Rémy Magné, Odile Bruneel, Dominique Buzoni-Gatel
(Affiliation: Université François-Rabelais de Tours, INRA, UMR 483 Université-INRA d'Immunologie Parasitaire et Vaccinologie, IFR Agents transmissibles et Infectiologie, UFR des Sciences Pharmaceutiques, 31 Avenue Monge, 37200 Tours, France. mevelec at univ-tours.fr)
|
| Journal | Vaccine
(Vaccine)
Vol. 23
Issue 36
Pg. 4489-99
(Aug 22 2005)
ISSN: 0264-410X Netherlands |
| PMID | 15935521
(Publication Type: Journal Article)
|
| Chemical References |
- Antigens, Protozoan
- GRA4 protein, Toxoplasma gondii
- Protozoan Proteins
- Protozoan Vaccines
- SAG1 antigen, Toxoplasma
- Vaccines, DNA
- Interleukin-4
- Interferon-gamma
- Granulocyte-Macrophage Colony-Stimulating Factor
|
| Topics |
- Animals
- Antigens, Protozoan
(genetics, immunology)
- Body Weight
- Female
- Granulocyte-Macrophage Colony-Stimulating Factor
(genetics)
- Interferon-gamma
(biosynthesis)
- Interleukin-4
(biosynthesis)
- Mice
- Mice, Inbred C57BL
- Plasmids
- Protozoan Proteins
(genetics, immunology)
- Protozoan Vaccines
(immunology)
- Toxoplasma
(immunology)
- Toxoplasmosis, Animal
(prevention & control)
- Toxoplasmosis, Congenital
(prevention & control)
- Vaccination
- Vaccines, DNA
(immunology)
|
