Flavoridin inhibits Yersinia enterocolitica uptake into fibronectin-adherent HeLa cells.

In this study, three structurally distinct disintegrins (flavoridin, echistatin, kistrin) were used as molecular probes to further characterize the molecular mechanisms underlying Yersinia enterocolitica infection of host cells. The activity of the three disintegrins on Y. enterocolitica uptake into fibronectin-adherent HeLa cells was evaluated at disintegrin doses which were non-cytotoxic and unable to induce cell detachment. Flavoridin resulted to be the most effective in inhibiting bacterial entry into host cells; echistatin was almost 50% less effective than flavoridin, whereas kistrin was definitely inactive. Our results suggest that alpha(5)beta(1) integrin receptor, which binds flavoridin with higher affinity than the other two disintegrins, plays a major role in Y. enterocolitica uptake into HeLa cells. Furthermore, flavoridin binding to this integrin prevented the disruption of the functional complex FAK-Cas, which occurs in the Y. enterocolitica uptake process.
AuthorsAntonio Scibelli, Gianluca Matteoli, Sante Roperto, Elena Alimenti, Ludovico Dipineto, Luigi Michele Pavone, Rossella Della Morte, Lucia Francesca Menna, Alessandro Fioretti, Norma Staiano
JournalFEMS microbiology letters (FEMS Microbiol Lett) Vol. 247 Issue 1 Pg. 51-7 (Jun 1 2005) ISSN: 0378-1097 [Print] Netherlands
PMID15927747 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Crotalid Venoms
  • Disintegrins
  • Fibronectins
  • Integrin alpha5beta1
  • Molecular Probes
  • Peptides
  • flavoridin protein, Trimeresurus
  • Focal Adhesion Kinase 1
  • PTK2 protein, human
  • Bacterial Adhesion (drug effects)
  • Crotalid Venoms (pharmacology)
  • Disintegrins (pharmacology)
  • Fibronectins
  • Focal Adhesion Kinase 1 (chemistry, metabolism)
  • HeLa Cells (drug effects, microbiology)
  • Humans
  • Integrin alpha5beta1 (chemistry, metabolism)
  • Molecular Probes
  • Peptides (pharmacology)
  • Yersinia enterocolitica (drug effects, pathogenicity)

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