Abstract | BACKGROUND: METHODS: Four groups of male Wistar rats were used: 1) control rats; 2) rats treated with L-NAME (35 mg/100 mL in drinking fluid); 3) rats treated with tempol (18 mg/100 mL in drinking fluid); and 4) rats treated with L-NAME plus tempol. All treatments were maintained for 6 weeks. Body weight, systolic blood pressure (BP) determined by the tail-cuff method, and heart rate were measured once per week. At the end of the experimental period, direct BP and morphologic, metabolic, plasma, and renal variables were measured. Enzymatic activities were measured in the kidney (cortex and medulla) and heart (right and left ventricles). RESULTS: Rats with L-NAME-induced hypertension showed increased copper- zinc (Cu-Zn) SOD activity in the renal cortex and medulla and the left and right ventricles, which was reduced by tempol administration. The manganese ( Mn) SOD activity was increased by L-NAME and reduced by tempol in the renal cortex but was unchanged in other tissues. Catalase activity was not affected by L-NAME or tempol treatments in any tissue. Both GPX and GR activities were increased by L-NAME and reduced by tempol in the renal cortex and medulla but were not affected in the ventricles. Tempol reduced BP and total urinary excretion of 8-hydroxy-2'-deoxyguanosine in L-NAME-treated animals but did not affect either variable in controls. CONCLUSIONS:
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Authors | Juan Sainz, Rosemary Wangensteen, Isabel Rodríguez Gómez, Juan Manuel Moreno, Virginia Chamorro, Antonio Osuna, Pablo Bueno, Félix Vargas |
Journal | American journal of hypertension
(Am J Hypertens)
Vol. 18
Issue 6
Pg. 871-7
(Jun 2005)
ISSN: 0895-7061 [Print] United States |
PMID | 15925750
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antioxidants
- Biomarkers
- Cyclic N-Oxides
- Enzyme Inhibitors
- Spin Labels
- Nitric Oxide
- 8-Hydroxy-2'-Deoxyguanosine
- Oxidoreductases
- Catalase
- Glutathione Peroxidase
- Superoxide Dismutase
- Glutathione Reductase
- Deoxyguanosine
- tempol
- NG-Nitroarginine Methyl Ester
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Topics |
- 8-Hydroxy-2'-Deoxyguanosine
- Animals
- Antioxidants
(therapeutic use)
- Biomarkers
(metabolism)
- Blood Pressure
(drug effects, physiology)
- Catalase
(drug effects, metabolism)
- Cyclic N-Oxides
(therapeutic use)
- Deoxyguanosine
(analogs & derivatives, urine)
- Disease Models, Animal
- Enzyme Inhibitors
(toxicity)
- Enzyme-Linked Immunosorbent Assay
- Glutathione Peroxidase
(drug effects, metabolism)
- Glutathione Reductase
(drug effects, metabolism)
- Heart Rate
(drug effects, physiology)
- Heart Ventricles
(enzymology, pathology)
- Hypertension
(chemically induced, drug therapy, enzymology)
- Kidney Cortex
(enzymology, pathology)
- Kidney Medulla
(enzymology, pathology)
- Male
- NG-Nitroarginine Methyl Ester
(toxicity)
- Nitric Oxide
(antagonists & inhibitors)
- Oxidative Stress
(drug effects)
- Oxidoreductases
(drug effects, metabolism)
- Rats
- Rats, Wistar
- Spectrophotometry
- Spin Labels
- Superoxide Dismutase
(drug effects, metabolism)
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