HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

An acetyl-CoA synthetase not encoded by the facA gene is expressed under carbon starvation in Phycomyces blakesleeanus.

Abstract
Two forms of acetyl-CoA synthetase (ACS1 and ACS2) have been detected in Phycomyces blakesleeanus. ACS1, encoded by the gene facA, was induced by acetate and repressed by glucose at the transcriptional level. ACS2, not encoded by the gene facA, was detected as a response to carbon starvation both in the wild type and in an facA(-) mutant. Both enzymes were purified and characterized. They can use acetate and propionate as substrates. ACS2 is a much more stable enzyme than ACS1. After 60 min incubation at 55 degrees C, ACS2 retained 50% of its activity whereas ACS1 only retained 3%. The optimum temperature was 50 degrees C for ACS2 and 30 degrees C for ACS1.
AuthorsSergio De Cima, Javier Rúa, Eusebio Perdiguero, Pilar del Valle, Félix Busto, Alberto Baroja-Mazo, Dolores de Arriaga
JournalResearch in microbiology (Res Microbiol) 2005 Jun-Jul Vol. 156 Issue 5-6 Pg. 663-9 ISSN: 0923-2508 [Print] France
PMID15921892 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Propionates
  • RNA, Fungal
  • RNA, Messenger
  • Coenzyme A Ligases
  • acetate-CoA ligase (ADP-forming)
  • propionic acid
  • Acetic Acid
Topics
  • Acetic Acid (metabolism)
  • Blotting, Northern
  • Coenzyme A Ligases (biosynthesis, genetics, isolation & purification)
  • Enzyme Stability
  • Gene Expression Regulation, Fungal
  • Hot Temperature
  • Kinetics
  • Phycomyces (enzymology)
  • Propionates (metabolism)
  • RNA, Fungal (analysis)
  • RNA, Messenger (analysis)
  • Substrate Specificity
  • Temperature
  • Time Factors

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: