Isolation and characterization of a Vicia graminea and Vicia unijuga lectins-binding (Vgu) glycoprotein with Thomsen-Friedenreich (T) activity from human liver metastases of pancreas carcinoma.

Abstract

1. Perchloric acid-soluble fraction from liver metastases of pancreas carcinoma of a patient with blood group B, was subjected to a systematic affinity chromatography using Vicia unijuga lectin (VUA) and Arachis hypogaea anti-T lectin (PNA) as immobilized ligands and separated into three fractions, B-active glycoprotein fraction, serologically inactive glycoprotein fraction and glycoprotein (VP) fraction exhibiting reactivities for Vicia graminea lectin (VGA), VUA and PNA. 2. VGA- and VUA-binding (Vgu) glycoprotein with Thomsen-Friedenreich (T) activity which was isolated, in a high state of purity, from VP fraction by HPLC using Asahipak GS-710 column, was demonstrated to be a mannose-rich glycoprotein with mol. wt of 1492 kDa and contained 40.40% carbohydrate.

• Authors: E Ueno, K Otsuka, T Kudo, S Ohkuma
• Journal: The International journal of biochemistry (Int J Biochem) Vol. 24 Issue 5 Pg. 769-76 (May 1992) ISSN: 0020-711X [Print] England
• PMID: 1592152 (Publication Type: Journal Article)

Chemical References

• Antigens, Tumor-Associated, Carbohydrate
• Disaccharides
• Glycoproteins
• Lectins
• Neoplasm Proteins
• Plant Lectins
• Vicia lectins
• Thomsen-Friedenreich antigen

Topics

• Antigens, Tumor-Associated, Carbohydrate
• Chromatography, Affinity
• Chromatography, High Pressure Liquid
• Disaccharides (isolation & purification)
• Glycoproteins (immunology, isolation & purification, metabolism)
• Humans
• Lectins (metabolism)
• Liver Neoplasms (metabolism, secondary)
• Molecular Weight
• Neoplasm Proteins (immunology, isolation & purification, metabolism)
• Pancreatic Neoplasms (pathology)
• Plant Lectins