| Abstract | OBJECTIVE: To observe the morphological and molecular biological peculiarities of the experimental autoimmune prostatitis (EAP) rat model made by SC purified prostate protein twice with immune adjuvant. METHODS: Male rats were intradermally immunized with a saline extract of male rat prostate glands (RPG) in Freund's complete adjuvant (FCA) and Pertussis-Diphtheria-Tetanus vaccine 0.5 ml i.p. at the 0 and 30th day, and the concentrations of the extract were respectively 5 mg/ml, 10 mg/ml and 15 mg/ml. At the 45th day, the rats were sacrificed and the morphological and molecular biological changes of the prostate specimens were observed to determine the effective concentration of RPG for a successful model. RESULTS: The expression of inflammation genes such as TNF-alpha, IL-1beta, IL-2 and iNOS obviously increased in the high-dosage model group; LM, EM and in situ hybridization revealed appearant chronic inflammation response, but this was not the case in the other two dosage groups. CONCLUSION: 15 mg/ml RPG mixed with FCA (1:1) 1.0 ml SC with Pertussis-Diphtheria-Tetanus vaccine 0.5 ml i.p. was an effective dosage for the successful model in our experiment. |
| Authors | Xiao-hui Zhou, Lei Han, Zhi-heng Zhou, Zhong-de Liu, Ji-xiang Yang, Yan-wei Lv, Chun-lai You
(Affiliation: Zhejiang University School of Pharmaceutical Sciences, Hangzhou, Zhejiang 310031, China. zhxh at zju.edu.cn)
|
| Journal | Zhonghua nan ke xue = National journal of andrology
(Zhonghua Nan Ke Xue)
Vol. 11
Issue 4
Pg. 290-5
(Apr 2005)
ISSN: 1009-3591 China |
| PMID | 15921263
(Publication Type: English Abstract, Journal Article)
|
| Chemical References |
- Diphtheria-Tetanus-Pertussis Vaccine
- Proteins
- Freund's Adjuvant
|
| Topics |
- Animals
- Autoimmune Diseases
(immunology, metabolism, pathology)
- Diphtheria-Tetanus-Pertussis Vaccine
(administration & dosage)
- Disease Models, Animal
- Freund's Adjuvant
(administration & dosage)
- Injections, Intraperitoneal
- Male
- Prostate
(metabolism, pathology)
- Prostatitis
(immunology, metabolism, pathology)
- Proteins
(administration & dosage)
- Rats
- Rats, Wistar
|
