Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types.

Abstract

BACKGROUND:

There are now several lines of evidence to suggest that protein synthesis and translation factors are involved in the regulation of cell proliferation and cancer development.

AIMS:

To investigate gene expression patterns of eukaryotic releasing factor 3 (eRF3) in gastric cancer.

METHODS:

RNA was prepared from 25 gastric tumour biopsies and adjacent non-neoplastic mucosa. Real time TaqMan reverse transcription polymerase chain reaction (RT-PCR) was performed to measure the relative gene expression levels. DNA was isolated from tumour and normal tissues and gene dosage was determined by a quantitative real time PCR using SYBR Green dye.

RESULTS:

Different histological types of gastric tumours were analysed and nine of the 25 tumours revealed eRF3/GSPT1 overexpression; moreover, eight of the 12 intestinal type carcinomas analysed overexpressed the gene, whereas eRF3/GSPT1 was overexpressed in only one of the 10 diffuse type carcinomas (Kruskal-Wallis Test; p < 0.05). No correlation was found between ploidy and transcript expression levels of eRF3/GSPT1. Overexpression of eRF3/GSPT1 was not associated with increased translation rates because the upregulation of eRF3/GSPT1 did not correlate with increased eRF1 levels.

CONCLUSIONS:

Overexpression of eRF3/GSPT1 in intestinal type gastric tumours may lead to an increase in the translation efficiency of specific oncogenic transcripts. Alternatively, eRF3/GSPT1 may be involved in tumorigenesis as a result of its non-translational roles, namely (dis)regulating the cell cycle, apoptosis, or transcription.

• Authors: J Malta-Vacas, C Aires, P Costa, A R Conde, S Ramos, A P Martins, C Monteiro, M Brito
• Journal: Journal of clinical pathology (J Clin Pathol) Vol. 58 Issue 6 Pg. 621-5 (Jun 2005) ISSN: 0021-9746 [Print] England
• PMID: 15917414 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm
• Neoplasm Proteins
• Peptide Termination Factors
• RNA, Neoplasm
• peptide-chain-release factor 3

Topics

• Adult
• Aged
• Aged, 80 and over
• DNA, Neoplasm (genetics)
• Gene Dosage
• Humans
• Middle Aged
• Neoplasm Proteins (genetics, metabolism)
• Peptide Termination Factors (genetics, metabolism)
• RNA, Neoplasm (genetics)
• Reverse Transcriptase Polymerase Chain Reaction (methods)
• Stomach Neoplasms (metabolism, pathology)
• Up-Regulation