Abstract |
The mechanisms underlying the pathogenicity of CCR5-restricted (R5) human immunodeficiency virus type-1 (HIV-1) strains are incompletely understood. Acquisition or enhancement of macrophage (M)-tropism by R5 viruses contributes to R5 HIV-1 pathogenesis. In this study, we show that M-tropic R5 viruses isolated from individuals with acquired immunodeficiency syndrome (late R5 viruses) require lower levels of CD4/CCR5 expression for entry, have decreased sensitivity to inhibition by the entry inhibitors TAK-779 and T-20, and have increased sensitivity to neutralization by the Env MAb IgG1b12 compared with non-M-tropic R5 viruses isolated from asymptomatic, immunocompetent individuals (early R5 viruses). Augmenting CCR5 expression levels on monocyte-derived macrophages via retroviral transduction led to a complete or marginal restoration of M-tropism by early R5 viruses, depending on the viral strain. Thus, reduced CD4/CCR5 dependence is a phenotype of R5 HIV-1 associated with M-tropism and late stage infection, which may affect the efficacy of HIV-1 entry inhibitors.
|
Authors | Lachlan Gray, Jasminka Sterjovski, Melissa Churchill, Philip Ellery, Najla Nasr, Sharon R Lewin, Suzanne M Crowe, Steven L Wesselingh, Anthony L Cunningham, Paul R Gorry |
Journal | Virology
(Virology)
Vol. 337
Issue 2
Pg. 384-98
(Jul 05 2005)
ISSN: 0042-6822 [Print] United States |
PMID | 15916792
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Antigens, CD
- CD4 Antigens
- Receptors, CCR5
- Receptors, HIV
|
Topics |
- Acquired Immunodeficiency Syndrome
(virology)
- Antigens, CD
(immunology)
- CD4 Antigens
(immunology)
- Cell Differentiation
- Cell Line
- HIV-1
(isolation & purification)
- Humans
- Kidney
- Macrophages
(physiology, virology)
- Monocytes
(cytology, virology)
- Receptors, CCR5
(physiology)
- Receptors, HIV
(physiology)
|