Abstract |
Human lymphocytes are resistant to genetic modification, particularly from recombinant adenoviruses, thus hampering the analysis of gene function using adenoviral vectors. This study engineered an Epstein-Barr virus-transformed B-lymphoblastoid cell line permissive to adenovirus infection and elucidated key roles for both the coxsackie-adenovirus receptor and alphavbeta5 integrin in mediating entry of adenoviruses into these cells. The work identified a strategy for engineering B cells to become susceptible to adenovirus infection and showed that such a strategy could be useful for the introduction of genes to alter lymphoblastoid-cell gene expression.
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Authors | Ciarán Richardson, Paul Brennan, Martin Powell, Stuart Prince, Yun-Hsiang Chen, O Brad Spiller, Martin Rowe |
Journal | The Journal of general virology
(J Gen Virol)
Vol. 86
Issue Pt 6
Pg. 1669-1679
(Jun 2005)
ISSN: 0022-1317 [Print] England |
PMID | 15914844
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CLMP protein, human
- Coxsackie and Adenovirus Receptor-Like Membrane Protein
- Integrins
- Receptors, Virus
- Receptors, Vitronectin
- integrin alphaVbeta5
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Topics |
- Adenovirus Infections, Human
(virology)
- Adenoviruses, Human
(metabolism, physiology)
- B-Lymphocytes
(metabolism, virology)
- Coxsackie and Adenovirus Receptor-Like Membrane Protein
- Humans
- Integrins
(metabolism)
- Receptors, Virus
(metabolism)
- Receptors, Vitronectin
(metabolism)
- Virus Replication
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