Abstract |
Varicella-zoster virus infects human dendritic cells (DCs). We found that VZV infection of DCs resulted in down-regulation of Fas expression on the surface of cells. VZV ORF47 was critical for replication of virus in human immature, but not mature DCs. Immature DCs infected with a mutant virus unable to express ORF47 expressed similar levels of a VZV immediate-early protein as cells infected with parental virus; however, cells infected with the ORF47 mutant expressed lower levels of glycoprotein E. Thus, in the absence of ORF47 protein, there is a block in viral replication between immediate-early and late gene expression. VZV unable to express ORF47 was severely impaired for spread of virus from DCs to melanoma cells. Infection of DCs with parental VZV resulted in a different pattern of phosphoproteins compared with the ORF47 mutant virus. Thus, VZV ORF47 is important for replication in immature DCs and for spread to other cells.
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Authors | Huiling Hu, Jeffrey I Cohen |
Journal | Virology
(Virology)
Vol. 337
Issue 2
Pg. 304-11
(Jul 05 2005)
ISSN: 0042-6822 [Print] United States |
PMID | 15913699
(Publication Type: Journal Article)
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Chemical References |
- Antigens, CD
- ORF47 protein, varicella-zoster virus
- Viral Proteins
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Topics |
- Antigens, CD
(blood)
- Cells, Cultured
- Chickenpox
(virology)
- Dendritic Cells
(microbiology, virology)
- Fibroblasts
(virology)
- Herpesvirus 3, Human
(genetics, physiology)
- Humans
- Monocytes
(microbiology, virology)
- Open Reading Frames
- Viral Proteins
(genetics)
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