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Varicella-zoster virus open reading frame 47 (ORF47) protein is critical for virus replication in dendritic cells and for spread to other cells.

Abstract
Varicella-zoster virus infects human dendritic cells (DCs). We found that VZV infection of DCs resulted in down-regulation of Fas expression on the surface of cells. VZV ORF47 was critical for replication of virus in human immature, but not mature DCs. Immature DCs infected with a mutant virus unable to express ORF47 expressed similar levels of a VZV immediate-early protein as cells infected with parental virus; however, cells infected with the ORF47 mutant expressed lower levels of glycoprotein E. Thus, in the absence of ORF47 protein, there is a block in viral replication between immediate-early and late gene expression. VZV unable to express ORF47 was severely impaired for spread of virus from DCs to melanoma cells. Infection of DCs with parental VZV resulted in a different pattern of phosphoproteins compared with the ORF47 mutant virus. Thus, VZV ORF47 is important for replication in immature DCs and for spread to other cells.
AuthorsHuiling Hu, Jeffrey I Cohen
JournalVirology (Virology) Vol. 337 Issue 2 Pg. 304-11 (Jul 05 2005) ISSN: 0042-6822 [Print] United States
PMID15913699 (Publication Type: Journal Article)
Chemical References
  • Antigens, CD
  • ORF47 protein, varicella-zoster virus
  • Viral Proteins
Topics
  • Antigens, CD (blood)
  • Cells, Cultured
  • Chickenpox (virology)
  • Dendritic Cells (microbiology, virology)
  • Fibroblasts (virology)
  • Herpesvirus 3, Human (genetics, physiology)
  • Humans
  • Monocytes (microbiology, virology)
  • Open Reading Frames
  • Viral Proteins (genetics)

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