Real-time RT-PCR (TaqMan) of tumor mRNA to predict sensitivity of specimens to 5-fluorouracil.

Abstract	5-Fluorouracil (5-FU) is still a key drug in the treatment of various kinds of advanced cancer, including breast and gastrointestinal carcinomas. To predict the sensitivity of colorectal cancer to 5-FU, mRNA is extracted from surgically obtained cancer specimens and expression of thymidylate synthetase (TS), dihydropyrimidine dehydrogenase (DPD), thymidine phosphorylase (TP), uridine phosphorylase (UP), es-nucleoside transporter (NT), and E2F1 are detected by real-time reverse transcription polymerase chain reaction (RT-PCR) (TaqMan). Previous results have shown that the catabolic rate-limiting enzymes DPD and NT, which are important membranous transporter of nucleosides, may regulate the sensitivity to 5-FU.
Authors	Tetsuro Kubota
Journal	Methods in molecular medicine (Methods Mol Med) Vol. 111 Pg. 257-65 ( 2005) ISSN: 1543-1894 [Print] United States
PMID	15911984 (Publication Type: Journal Article)
Chemical References	Antimetabolites, Antineoplastic Antineoplastic Agents Cell Cycle Proteins DNA Primers DNA-Binding Proteins E2F Transcription Factors E2F1 Transcription Factor E2F1 protein, human Nucleoside Transport Proteins RNA, Messenger RNA, Neoplasm Transcription Factors Dihydrouracil Dehydrogenase (NADP) Thymidylate Synthase Uridine Phosphorylase Thymidine Phosphorylase Fluorouracil
Topics	Antimetabolites, Antineoplastic (pharmacology) Antineoplastic Agents (pharmacology) Cell Cycle Proteins (metabolism) Cell Membrane (metabolism) Colorectal Neoplasms (drug therapy) DNA Primers (chemistry) DNA-Binding Proteins (metabolism) Dihydrouracil Dehydrogenase (NADP) (metabolism) Drug Screening Assays, Antitumor E2F Transcription Factors E2F1 Transcription Factor Fluorouracil (pharmacology) Humans Nucleoside Transport Proteins (metabolism) Polymerase Chain Reaction RNA, Messenger (metabolism) RNA, Neoplasm (chemistry, metabolism) Reverse Transcriptase Polymerase Chain Reaction (methods) Sensitivity and Specificity Thymidine Phosphorylase (metabolism) Thymidylate Synthase (metabolism) Transcription Factors (metabolism) Uridine Phosphorylase (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!