AIDS-related B-cell lymphoma (ARL): correlation of prognosis with differentiation profiles assessed by immunophenotyping.

Abstract

This study was undertaken to analyze the differentiation profiles assessed by immunophenotyping in AIDS-related B-cell lymphoma (ARL) and their relation to the clinical course. Paraffin-embedded sections of 89 ARL cases during 1989 to 2004 were stained immunohistochemically with antibodies to CD3, CD10, CD20, CD38, CD138/Syndecan-1 (Syn-1), multiple myeloma-1/interferon regulatory factor-4 (MUM1/IRF4), B-cell lymphoma protein-2 (BCL-2), BCL-6, latent membrane protein-1 (LMP-1), and Ki-67. Expression of CD10 and CD20 were associated with better overall survival (OS; P = .009 and P = .04, respectively). Expression of CD20 was associated with longer disease-free survival (DFS; P = .03), whereas expression of CD138/Syn-1 was associated with shorter DFS (P = .03). OS and DFS were worse in patients with immunophenotypic profiles related to post-germinal center (GC) differentiation (BCL-6 and CD10 negative, MUM1/IRF4 and/or CD138/Syn-1 positive) when compared with GC differentiation (P = .01). When controlled for age-adjusted International Prognostic Index (IPI), prior AIDS-defining illness (ADI), and year of ARL diagnosis, a post-GC differentiation remained significantly associated with poor OS and DFS. Expression of CD10 was associated with a preserved immunocompetence, whereas CD20 was less frequent in patients developing ARL while on highly active antiretroviral therapy (P = .04). In summary, lack of CD20 or CD10 expression and a post-germinal center signature are associated with a worse prognosis in ARL.

• Authors: Christian Hoffmann, Markus Tiemann, Carsten Schrader, Dirk Janssen, Eva Wolf, Mathias Vierbuchen, Reza Parwaresch, Karen Ernestus, Andreas Plettenberg, Albrecht Stoehr, Gerd Fatkenheuer, Christoph Wyen, Mark Oette, Heinz-August Horst
• Journal: Blood (Blood) Vol. 106 Issue 5 Pg. 1762-9 (Sep 01 2005) ISSN: 0006-4971 [Print] United States
• PMID: 15905193 (Publication Type: Clinical Trial, Journal Article)

Chemical References

• Antigens, CD
• Antigens, CD20
• Antigens, Differentiation
• Biomarkers, Tumor
• Neprilysin

Topics

• Adult
• Aged
• Antigens, CD (biosynthesis, immunology)
• Antigens, CD20 (biosynthesis, immunology)
• Antigens, Differentiation (biosynthesis, immunology)
• Biomarkers, Tumor (analysis)
• Disease-Free Survival
• Female
• Follow-Up Studies
• Germinal Center (immunology)
• Humans
• Immunohistochemistry
• Immunophenotyping (methods)
• Lymphoma, AIDS-Related (diagnosis, immunology)
• Male
• Middle Aged
• Neprilysin (biosynthesis, immunology)
• Prognosis
• Regression Analysis
• Reproducibility of Results
• Retrospective Studies
• Survival Analysis
• Time Factors