Abnormal expression of neurofilament proteins in Ewing's sarcoma cell cultures.

Abstract

A neural origin of Ewing's sarcoma (ES) has often been suggested and we have demonstrated neurofilament protein expression in ES cells. However, only the 200-kD subunit has been revealed in all of the ES cells analyzed. The 160- and 68-kD subunits were always absent. For these reasons, we have attempted to induce neural differentiation in 3 ES cell lines with different types of inducers: tetradecanoylphorbol-13-acetate (TPA) retinoic acid and nerve growth factor. When the cell lines were cultured for 7 days with TPA (10(-9) M) or retinoic acid (10(-7) M), only the 68-kD neurofilament subunit was slightly induced. No inducation was obtained when nerve growth factor was used, even at a 21-day culture. These results are in agreement with the putative neural origin of ES and may indicate an abnormal expression of neurofilament proteins in this tumor.

• Authors: S Lizard-Nacol, C Volk, G Lizard, C Turc-Carel
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 13 Issue 1-2 Pg. 36-43 ( 1992) ISSN: 1010-4283 [Print] Netherlands
• PMID: 1589695 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Neurofilament Proteins
• Tretinoin
• Tetradecanoylphorbol Acetate

Topics

• Cell Division (drug effects)
• Cell Line
• Flow Cytometry
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Microscopy, Electron
• Neurofilament Proteins (biosynthesis)
• Sarcoma, Ewing (metabolism, pathology)
• Tetradecanoylphorbol Acetate
• Tretinoin (pharmacology)
• Tumor Cells, Cultured