Identification and characterization of HIV-1-specific CD8+ T cell epitopes presented by HLA-A*2601.

Abstract

Since HLA-A*26 is one of the most common alleles in Asia, where approximately 20% of people have this allele, identification of HIV-1-specific epitopes presented by HLA-A*26 is necessary for studies on the immunopathogenesis of AIDS and vaccine development in Asia. As presented herein, we used the reverse immunogenetics approach to identify HIV-1 epitopes presented by HLA-A*2601, one of the major HLA-A*26 subtypes. We selected 24 HLA-A*2601-binding peptides out of 110 HIV-1 peptides by using a HLA-A*2601 stabilization assay. The ability of these HLA-A*2601-binding peptides to induce peptide-specific CD8(+) T cells was tested by stimulating PBMCs from HIV-1-infected individuals having HLA-A*2601 with these peptides. Four HLA-A*2601-binding peptides induced peptide-specific CD8 T cells. Analysis using HIV-1 recombinant vaccinia-infected C1R-A*2601 cells indicated that these four peptides were HIV-1 epitopes endogenously presented by HLA-A*2601. Two epitope-specific CD8(+) T cells were predominantly detected in HIV-1 infected individuals, suggesting that these epitopes may be useful for vaccine development.

• Authors: Manami Satoh, Yuji Takamiya, Shinichi Oka, Katsushi Tokunaga, Masafumi Takiguchi
• Journal: Vaccine (Vaccine) Vol. 23 Issue 29 Pg. 3783-90 (May 31 2005) ISSN: 0264-410X [Print] Netherlands
• PMID: 15893615 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Epitopes, T-Lymphocyte
• HLA-A Antigens
• Peptides
• Interferon-gamma

Topics

• Animals
• Antigens, Viral (immunology)
• CD8-Positive T-Lymphocytes (immunology)
• Cell Line
• Cells, Cultured
• Cytotoxicity Tests, Immunologic
• Epitopes, T-Lymphocyte (chemistry, immunology)
• HIV Infections (immunology, virology)
• HIV-1 (immunology)
• HLA-A Antigens (immunology)
• Humans
• Interferon-gamma (analysis)
• Mice
• Peptides (chemical synthesis, chemistry, immunology)
• Protein Binding