A new Hypocrea strain producing harzianum A cytotoxic to tumour cell lines.

Abstract

AIMS:

To identify a new fungal strain, Hypocrea sp. F000527 producing a trichothecene metabolite, harzianum A, and to evaluate its cytotoxicity to tumour cell lines.

METHODS AND RESULTS:

A fungal strain, F000527, with cytotoxic activity was identified as a new Hypocrea strain based on morphological characteristics and internal transcribed spacers rDNA sequence data. Harzianum A was isolated from wheat bran culture by 50% acetone extraction, silica gel chromatography, Sephadex LH-20 chromatography and HPLC. The chemical structures were determined by ESI- or HRFAB-MS and (1)H and (13)C-NMR analyses. Harzianum A showed cytotoxicity to HT1080 and HeLa cell lines with IC(50) value of 0.65 and 5.07 Łg ml(-1) respectively.

CONCLUSIONS:

Harzianum A with a chemical formula of C(23)H(28)O(6) was isolated from a new Hypocrea strain and showed moderate to strong cytotoxicity to human cancer cell lines.

SIGNIFICANCE AND IMPACT OF THE STUDY:

This is the first report of the production of cytotoxic harzianum A by a new Hypocrea strain.

• Authors: H B Lee, Y Kim, H Z Jin, J J Lee, C-J Kim, J Y Park, H S Jung
• Journal: Letters in applied microbiology (Lett Appl Microbiol) Vol. 40 Issue 6 Pg. 497-503 ( 2005) ISSN: 0266-8254 [Print] England
• PMID: 15892749 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• DNA, Fungal
• DNA, Ribosomal
• Dietary Fiber
• Trichothecenes
• harzianum A

Topics

• Antineoplastic Agents (isolation & purification, metabolism, pharmacology)
• Cell Line, Tumor
• Chromatography
• DNA, Fungal (chemistry)
• DNA, Ribosomal (chemistry)
• Dietary Fiber (microbiology)
• Humans
• Hypocrea (classification, isolation & purification, metabolism)
• Inhibitory Concentration 50
• Korea
• Magnetic Resonance Spectroscopy
• Phylogeny
• Sequence Analysis, DNA
• Spectrometry, Mass, Electrospray Ionization
• Spectrometry, Mass, Fast Atom Bombardment
• Trichothecenes (biosynthesis, isolation & purification, pharmacology)